Journal article
Anti-interleukin-6 antibody treatment restores cell-mediated immune function in mice with acute ethanol exposure before burn trauma
Alcoholism, clinical and experimental research, Vol.24(9), pp.1392-1399
2000
DOI: 10.1111/j.1530-0277.2000.tb02108.x
PMID: 11003205
Abstract
Background: Previous studies from this laboratory reported that suppression of cell-mediated immune function was coincident with elevated interleukin (IL)-6 production after acute ethanol exposure before burn trauma, compared with either insult alone. The goal of this study was to investigate whether treatment with an anti-IL-6 antibody could restore immunocompetence in mice subjected to burn trauma with previous exposure to alcohol, as assessed by delayed-type hypersensitivity (DTH) and mitogen-induced splenocyte proliferative responses.
Methods: Mice given an ethanol treatment designed to reach a blood alcohol level of 100 mg/dl before a 15% total body surface area burn injury were treated with an anti-IL-6 antibody at 30 min and 24 hr postinjury.
Results: Burn/ethanol mice exhibited a 91% suppression of the DTH response ( < 0.01) and a 76% suppression of mitogen-induced splenocyte proliferation (p < 0.01) at 48 hr postinjury, along with increased levels of circulating and splenic macrophage-derived IL-6, compared with all other treatment groups. After anti-IL-6 antibody administration to burn/ethanol mice, there was a 25% (p < 0.05) and 63% (p < 0.01) recovery of the DTH and splenocyte proliferative responses, respectively. Addition of exogenous IL-6 to splenocyte cultures isolated from anti-IL-6 antibody-treated burn/ethanol mice resulted in a 70% inhibition of mitogen-induced proliferative responses (p < 0.03).
Conclusions: These data confirm previous findings that burn in combination with acute ethanol exposure suppresses cell-mediated immune function compared with either insult alone. Furthermore, the ability of the anti-IL-6 antibody treatment to improve cellular immune responses in the burn/ethanol group suggests that blocking this cytokine may be beneficial for the ethanol-exposed, thermally injured individual.
Details
- Title: Subtitle
- Anti-interleukin-6 antibody treatment restores cell-mediated immune function in mice with acute ethanol exposure before burn trauma
- Creators
- Christine V FONTANILLA - Department of Cell Biology, Neurobiology, and Anatomy, the Bum and Shock Trauma Institute, Loyola University Medical Center, Maywood, Illinois, United StatesDouglas E FAUNCE - Department of Cell Biology, Neurobiology, and Anatomy, the Bum and Shock Trauma Institute, Loyola University Medical Center, Maywood, Illinois, United StatesMeredith S GREGORY - Department of Cell Biology, Neurobiology, and Anatomy, the Bum and Shock Trauma Institute, Loyola University Medical Center, Maywood, Illinois, United StatesKelly A. N MESSINGHAM - Department of Cell Biology, Neurobiology, and Anatomy, the Bum and Shock Trauma Institute, Loyola University Medical Center, Maywood, Illinois, United StatesElizabeth A DURBIN - Department of Cell Biology, Neurobiology, and Anatomy, the Bum and Shock Trauma Institute, Loyola University Medical Center, Maywood, Illinois, United StatesLisa A DUFFNER - Department of Cell Biology, Neurobiology, and Anatomy, the Bum and Shock Trauma Institute, Loyola University Medical Center, Maywood, Illinois, United StatesElizabeth J KOVACS - Department of Surgery, and Immunology and Aging Program, Loyola University Medical Center, Maywood, Illinois, United States
- Resource Type
- Journal article
- Publication Details
- Alcoholism, clinical and experimental research, Vol.24(9), pp.1392-1399
- DOI
- 10.1111/j.1530-0277.2000.tb02108.x
- PMID
- 11003205
- NLM abbreviation
- Alcohol Clin Exp Res
- ISSN
- 0145-6008
- eISSN
- 1530-0277
- Publisher
- Wiley; Baltimore, MD
- Language
- English
- Date published
- 2000
- Academic Unit
- Dermatology
- Record Identifier
- 9984025402102771
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