Antibiotic Augmentation of Thermal Eradication of Staphylococcus epidermidis Biofilm Infections

Haydar A S Aljaafari; Nadia I Abdulwahhab; Eric Nuxoll

doi:10.3390/pathogens13040327

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Antibiotic Augmentation of Thermal Eradication of Staphylococcus epidermidis Biofilm Infections

Journal article

Open access

Peer reviewed

Antibiotic Augmentation of Thermal Eradication of Staphylococcus epidermidis Biofilm Infections

Haydar A S Aljaafari, Nadia I Abdulwahhab and Eric Nuxoll

Pathogens (Basel), Vol.13(4), 327

04/16/2024

DOI: 10.3390/pathogens13040327

PMCID: PMC11054983

PMID: 38668282

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Abstract

is a major contributor to bacterial infections on medical implants, currently treated by surgical removal of the device and the surrounding infected tissue at considerable morbidity and expense. In situ hyperthermia is being investigated as a non-invasive means of mitigating these bacterial biofilm infections, but minimizing damage to the surrounding tissue requires augmenting the thermal shock with other approaches such as antibiotics and discerning the minimum shock required to eliminate the biofilm. biofilms were systematically shocked at a variety of temperatures (50-80 °C) and durations (1-10 min) to characterize their thermal susceptibility and compare it to other common nosocomial pathogens such as and . Biofilms were also exposed to three classes of antibiotics (ciprofloxacin, tobramycin and erythromycin) separately at concentrations ranging from 0 to 128 μg mL to evaluate their impact on the efficacy of thermal shock and the subsequent potential regrowth of the biofilm. biofilms were shown to be more thermally susceptible to hyperthermia than other common bacterial pathogens. All three antibiotics substantially decreased the duration and/or temperature needed to eliminate the biofilms, though this augmentation did not meet the criteria of synergism immediately following thermal shock. Subsequent reincubation, however, revealed strong synergism on a longer timescale.

Antibiotics

Hyperthermia

Staphylococcus epidermidis

thermal shock

biofilm

prothesis-related infections

Details

Title: Subtitle: Antibiotic Augmentation of Thermal Eradication of Staphylococcus epidermidis Biofilm Infections
Creators: Haydar A S Aljaafari - Department of Chemical Engineering, University of Technology, Baghdad 10066, Iraq
Nadia I Abdulwahhab - Department of Applied Sciences, University of Technology, Baghdad 10066, Iraq
Eric Nuxoll - University of Iowa
Resource Type: Journal article
Publication Details: Pathogens (Basel), Vol.13(4), 327
DOI: 10.3390/pathogens13040327
PMID: 38668282
PMCID: PMC11054983
NLM abbreviation: Pathogens
ISSN: 2076-0817
eISSN: 2076-0817
Grant note: 18IPA34170108 / American Heart Association CBET-1133297 / National Science Foundation
Language: English
Date published: 04/16/2024
Academic Unit: Chemical and Biochemical Engineering
Record Identifier: 9984618624002771

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