Journal article
Antibodies against the Calcium-Binding Protein: Calsequestrin from
Plant physiology (Bethesda), Vol.91(4), pp.1259-1261
12/1989
DOI: 10.1104/pp.91.4.1259
PMID: 16667172
Abstract
Plant microsomes contain a protein clearly related to a calcium-binding protein, calsequestrin, originally found in the sarcoplasmic reticulum of muscle cells, responsible for the rapid release and uptake of Ca
2+
within the cells. The location and role of calsequestrin in plant cells is unknown. To generate monoclonal antibodies specific to plant calsequestrin, mice were immunized with a microsomal fraction from cultured cells of
Streptanthus tortuosus
(Brassicaceae). Two clones cross-reacted with one protein band with a molecular weight equal to that of calsequestrin (57 kilodaltons) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. This band is able to bind
45
Ca
2+
and can be recognized by a polyclonal antibody against the canine cardiac muscle calsequestrin. Rabbit skeletal muscle calsequestrin cross-reacted with the plant monoclonal antibodies. The plant monoclonal antibodies generated here are specific to calsequestrin protein.
Details
- Title: Subtitle
- Antibodies against the Calcium-Binding Protein: Calsequestrin from
- Creators
- Mei Chou - Department of Botany, The University of Iowa, Iowa City, Iowa 52242Karl-Heinz Krause - Department of Botany, The University of Iowa, Iowa City, Iowa 52242Kevin P Campbell - Department of Botany, The University of Iowa, Iowa City, Iowa 52242Kenneth G Jensen - Department of Botany, The University of Iowa, Iowa City, Iowa 52242Richard D Sjolund - Department of Botany, The University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Plant physiology (Bethesda), Vol.91(4), pp.1259-1261
- DOI
- 10.1104/pp.91.4.1259
- PMID
- 16667172
- NLM abbreviation
- Plant Physiol
- ISSN
- 0032-0889
- eISSN
- 1532-2548
- Language
- English
- Date published
- 12/1989
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984068360202771
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