Journal article
Antibody characterization is critical to enhance reproducibility in biomedical research
eLife, Vol.13, e100211
08/14/2024
DOI: 10.7554/eLife.100211
PMCID: PMC11324233
PMID: 39140332
Abstract
Antibodies are used in many areas of biomedical and clinical research, but many of these antibodies have not been adequately characterized, which casts doubt on the results reported in many scientific papers. This problem is compounded by a lack of suitable control experiments in many studies. In this article we review the history of the 'antibody characterization crisis', and we document efforts and initiatives to address the problem, notably for antibodies that target human proteins. We also present recommendations for a range of stakeholders - researchers, universities, journals, antibody vendors and repositories, scientific societies and funders - to increase the reproducibility of studies that rely on antibodies.Antibodies are used in many areas of biomedical and clinical research, but many of these antibodies have not been adequately characterized, which casts doubt on the results reported in many scientific papers. This problem is compounded by a lack of suitable control experiments in many studies. In this article we review the history of the 'antibody characterization crisis', and we document efforts and initiatives to address the problem, notably for antibodies that target human proteins. We also present recommendations for a range of stakeholders - researchers, universities, journals, antibody vendors and repositories, scientific societies and funders - to increase the reproducibility of studies that rely on antibodies.
Details
- Title: Subtitle
- Antibody characterization is critical to enhance reproducibility in biomedical research
- Creators
- Richard A Kahn - Emory UniversityHarvinder Virk - University of LeicesterCarl Laflamme - Montreal Neurological Institute and HospitalDouglas W Houston - University of IowaNicole K Polinski - Michael J. Fox FoundationRob Meijers - Institute for Protein InnovationAllan I Levey - Emory UniversityClifford B Saper - Hadassah Medical CenterTimothy M Errington - Center for Open ScienceRachel E Turn - Stanford UniversityAnita Bandrowski - University of California, San DiegoJames S Trimmer - University of California, DavisMeghan Rego - AddgeneLeonard P Freedman - Frederick National Laboratory for Cancer Researchtunato FerraraAndrew R M BradburyHannah Cable - AbcamSkye Longworth
- Resource Type
- Journal article
- Publication Details
- eLife, Vol.13, e100211
- DOI
- 10.7554/eLife.100211
- PMID
- 39140332
- PMCID
- PMC11324233
- ISSN
- 2050-084X
- eISSN
- 2050-084X
- Language
- English
- Date published
- 08/14/2024
- Academic Unit
- Biology
- Record Identifier
- 9984697058602771
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