Antibody-mediated protection against cytotoxic T-cell escape in coronavirus-induced demyelination

Ajai A Dandekar; Gary Jacobsen; Thomas J Waldschmidt; Stanley Perlman

doi:10.1128/JVI.77.22.11867-11874.2003

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Antibody-mediated protection against cytotoxic T-cell escape in coronavirus-induced demyelination

Journal article

Open access

Peer reviewed

Antibody-mediated protection against cytotoxic T-cell escape in coronavirus-induced demyelination

Ajai A Dandekar, Gary Jacobsen, Thomas J Waldschmidt and Stanley Perlman

Journal of virology, Vol.77(22), pp.11867-11874

11/2003

DOI: 10.1128/JVI.77.22.11867-11874.2003

PMCID: PMC254260

PMID: 14581523

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https://europepmc.org/articles/pmc254260View

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Abstract

C57BL/6 (B6) mice infected with mouse hepatitis virus (MHV) strain JHM develop a clinically evident, demyelinating encephalomyelitis. Infectious virus can be isolated from the spinal cords of these mice and is invariably mutated in the immunodominant CD8 T-cell epitope recognized in this strain. We showed previously that these persistently infected mice did not mount a measurable serum anti-MHV neutralizing antibody response. Here we show that cytotoxic T-lymphocyte (CTL) escape was not detected in MHV-infected BALB/b mice (H-2(b) haplotype), even though the same CD8 T-cell epitopes were recognized as in B6 mice. BALB/b mice had 25-fold more MHV-specific antibody-secreting cells in the central nervous system, the site of infection, than B6 mice, suggesting that local production of anti-MHV antibody contributed to this absence of CTL escape. Additionally, administration of anti-MHV neutralizing antibody to infected B6 mice suppressed the development of CTL escape mutants. These findings indicate a key role for the anti-MHV antibody response in suppressing virus replication, thereby minimizing the emergence and competitive advantage of CTL escape mutants.

T-Lymphocytes, Cytotoxic - immunology

Animals

Antibody-Producing Cells - physiology

B-Lymphocytes - immunology

Spleen - immunology

Demyelinating Diseases - immunology

Immunization, Passive

Antibodies, Viral - immunology

Mice

Mice, Inbred BALB C

Brain - immunology

Murine hepatitis virus

Details

Title: Subtitle: Antibody-mediated protection against cytotoxic T-cell escape in coronavirus-induced demyelination
Creators: Ajai A Dandekar - Interdisciplinary Program in Immunology, University of Iowa, Iowa City, Iowa 52242, USA
Gary Jacobsen
Thomas J Waldschmidt
Stanley Perlman
Resource Type: Journal article
Publication Details: Journal of virology, Vol.77(22), pp.11867-11874
DOI: 10.1128/JVI.77.22.11867-11874.2003
PMID: 14581523
PMCID: PMC254260
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: United States
Grant note: NS36592 / NINDS NIH HHS F31 NS042981 / NINDS NIH HHS NS42981 / NINDS NIH HHS T32 GM007337 / NIGMS NIH HHS R01 NS036592 / NINDS NIH HHS
Language: English
Date published: 11/2003
Academic Unit: Preventive and Community Dentistry; Microbiology and Immunology; Stead Family Department of Pediatrics; Pathology; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
Record Identifier: 9983777477302771

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