Journal article
Antibody response of mice to lactate dehydrogenase-elevating virus during infection and immunization with inactivated virus
Virus research, Vol.5(4), pp.357-375
1986
DOI: 10.1016/0168-1702(86)90029-8
PMID: 3022497
Abstract
BALB/c and Swiss mice were infected with lactate dehydrogenase-elevating virus (LDV) or immunized with glutaraldehyde-inactivated or ether-extracted virus and their plasma was monitored for anti-LDV IgG and IgM levels by ELISA and indirect fluorescent antibody staining, for neutralizing antibodies, for sensitized antibody-virus complexes, for immune complexes, and for total plasma IgG and IgM. In infected mice, anti-LDV IgM was transiently formed during the first 2 weeks post infection (p.i.) but only at a low level. Anti-LDV IgG was produced in a biphasic manner with an initial peak at about 10 days p.i. and a secondary rise reaching a maximum level 30–80 days p.i. which was retained throughout the persistent phase of infection. The concomitant appearance of comparable levels of low molecular weight immune complexes suggests that most anti-LDV IgG was complexed with LDV proteins. Also, as early as 10 days p.i., infectious antibody-LDV complexes developed, which were neutralizable by rabbit anti-mouse IgG, whereas antibodies that neutralize the infectivity of exogenously added LDV appeared only 1–2 months p.i. Throughout infection, most of the anti-LDV IgG was directed to VP-3, the envelope glycoprotein of LDV, which was found to exist in at least 10 distinct forms ranging in molecular weight from 24 to 42 kDa. Anti-LDV IgG levels as high as those observed in infected mice developed in mice immunized with inactivated LDV. Antibodies to glutaraldehyde-inactivated LDV were also mainly directed to VP-3, but exhibited no neutralizing activity. The polyclonal B cell activation associated with a persistent LDV infection and the formation of immune complexes were not observed in mice immunized with inactivated virus.
Details
- Title: Subtitle
- Antibody response of mice to lactate dehydrogenase-elevating virus during infection and immunization with inactivated virus
- Creators
- William A CafrunyStephen P.K ChanJohn T HartyShida YousefiKatherine KowalchykDavid McDonaldBlair ForemanGreta BudwegPeter G.W Plagemann
- Resource Type
- Journal article
- Publication Details
- Virus research, Vol.5(4), pp.357-375
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0168-1702(86)90029-8
- PMID
- 3022497
- ISSN
- 0168-1702
- eISSN
- 1872-7492
- Language
- English
- Date published
- 1986
- Academic Unit
- Pathology
- Record Identifier
- 9984047745802771
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