Journal article
Antifungal Metabolites (Monorden, Monocillin IV, and Cerebrosides) from Humicola fuscoatra Traaen NRRL 22980, a Mycoparasite of Aspergillus flavus Sclerotia
Applied and environmental microbiology, Vol.64(11), pp.4482-4484
11/1998
DOI: 10.1128/aem.64.11.4482-4484.1998
PMCID: PMC106672
PMID: 9797310
Abstract
The mycoparasite
Humicola fuscoatra
NRRL 22980 was isolated from a sclerotium of
Aspergillus flavus
that had been buried in a cornfield near Tifton, Ga. When grown on autoclaved rice, this fungus produced the antifungal metabolites monorden, monocillin IV, and a new monorden analog. Each metabolite produced a clear zone of inhibition surrounding paper assay disks on agar plates seeded with conidia of
A. flavus
. Monorden was twice as inhibitory to
A. flavus
mycelium extension (MIC > 28 μg/ml) as monocillin IV (MIC > 56 μg/ml). Cerebrosides C and D, metabolites known to potentiate the activity of cell wall-active antibiotics, were separated from the ethyl acetate extract but were not inhibitory to
A. flavus
when tested as pure compounds. This is the first report of natural products from
H. fuscoatra
.
Details
- Title: Subtitle
- Antifungal Metabolites (Monorden, Monocillin IV, and Cerebrosides) from Humicola fuscoatra Traaen NRRL 22980, a Mycoparasite of Aspergillus flavus Sclerotia
- Creators
- Donald T Wicklow - Bioactive Agents Research, National Center for Agricultural Utilization Research, REE, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, and Department of Chemistry, University of Iowa, Iowa City, Iowa 52242Biren K Joshi - Bioactive Agents Research, National Center for Agricultural Utilization Research, REE, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, and Department of Chemistry, University of Iowa, Iowa City, Iowa 52242William R Gamble - Bioactive Agents Research, National Center for Agricultural Utilization Research, REE, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, and Department of Chemistry, University of Iowa, Iowa City, Iowa 52242James B Gloer - Bioactive Agents Research, National Center for Agricultural Utilization Research, REE, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, and Department of Chemistry, University of Iowa, Iowa City, Iowa 52242Patrick F Dowd - Bioactive Agents Research, National Center for Agricultural Utilization Research, REE, Agricultural Research Service, U.S. Department of Agriculture, Peoria, Illinois 61604, and Department of Chemistry, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Applied and environmental microbiology, Vol.64(11), pp.4482-4484
- Publisher
- American Society for Microbiology
- DOI
- 10.1128/aem.64.11.4482-4484.1998
- PMID
- 9797310
- PMCID
- PMC106672
- ISSN
- 0099-2240
- eISSN
- 1098-5336
- Language
- English
- Date published
- 11/1998
- Academic Unit
- Chemistry
- Record Identifier
- 9984216710102771
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