Journal article
Antifungal dynamics of LY 303366, an investigational echinocandin B analog, against Candida ssp
Diagnostic microbiology and infectious disease, Vol.26(3), pp.125-131
1996
DOI: 10.1016/S0732-8893(96)00202-7
PMID: 9078447
Abstract
Two isolates each of
Candida albicans, Candida tropicalis, and
Candida glabrata were selected for time-kill curve testing against LY 303366 at concentrations ranging from 0.125 × MIC to 16 × MIC. RPMI 1640 buffered with morpholinepropanesulfonic acid (MOPS) was utilized as growth medium. Samples were obtained at predetermined time points over 24 hours and streaked for colony count determination. Against
C. albicans (one strain) and
C. glabrata isolates, LY 303366 exhibited fungicidal (≥three log
10 reduction in CFU) activity. In contrast, fungistatic activity was observed with LY 303366 against
C. albicans (one strain) and
C. tropicalis isolates at all of the multiples of the MIC tested. With the exception of one
C. glabrata strain, the rate and extent of activity against test isolates was not enhanced with concentrations exceeding the MIC. Our data indicate that maximal antifungal activity with LY 303366 may be achieved by optimizing the time of fungal exposure to the drug. Additionally, these data suggest that use of the current interpretive endpoint for MICs in RPMI may underestimate the antifungal activity of LY 303366. Thus, the MIC endpoint may need to be re-evaluated, or perhaps an alternative media, such as antibiotic medium #3, should be utilized for determination of LY 303366 MICs.
Details
- Title: Subtitle
- Antifungal dynamics of LY 303366, an investigational echinocandin B analog, against Candida ssp
- Creators
- Michael E. Ernst - University of IowaMichael E. Klepser - University of IowaErika J. Wolfe - University of IowaMichael A. Pfaller - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.26(3), pp.125-131
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S0732-8893(96)00202-7
- PMID
- 9078447
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 1996
- Academic Unit
- Epidemiology; Pathology; Family and Community Medicine; Pharmacy Practice and Science
- Record Identifier
- 9984297455002771
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