Journal article
Antigen-based heteropolymers facilitate, via primate erythrocyte complement receptor type 1, rapid erythrocyte binding of an autoantibody and its clearance from the circulation in rhesus monkeys
The Journal of immunology (1950), Vol.155(1), pp.339-347
07/01/1995
DOI: 10.4049/jimmunol.155.1.339
PMID: 7602110
Abstract
We investigated the feasibility of using the primate E complement receptor (CR1), in concert with Ag-based heteropolymers (AHP), as a potential therapy to remove autoantibodies from the circulation. AHP are prepared by cross-linking an anti-CR1 mAb with the acetylcholine receptor (AChR), the principal target Ag in myasthenia gravis. In vitro studies demonstrate that this methodology facilitates specific, rapid, and quantitative binding of an anti-AChR mAb to primate Es. In vivo experiments in rhesus monkeys indicate that AHP-mediated binding of an anti-AChR mAb to Es leads to the clearance of the mAb from the circulation. Once bound to the E via the AHP, the autoantibody is transported to the liver and spleen, where it is degraded without destruction of the E. It is therefore likely that the complexes of AHP and target mAb, when bound to Es, are recognized in vivo and processed by a mechanism quite similar to that which occurs when complement-opsonized immune complexes, bound to primate Es, are cleared from the circulation. It may be possible to extend and generalize this work to allow for the development of a simple, noninvasive therapy that can be made specific for the treatment of several different autoimmune diseases.
Details
- Title: Subtitle
- Antigen-based heteropolymers facilitate, via primate erythrocyte complement receptor type 1, rapid erythrocyte binding of an autoantibody and its clearance from the circulation in rhesus monkeys
- Creators
- Polly J Ferguson - Department of Pediatrics, University of Virginia School of Medicine, Charlottesville 22908, USAEdward N MartinKirsten L GreeneSusan KuhnDavid S CafisoGeorge AddonaRonald P Taylor
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.155(1), pp.339-347
- Publisher
- United States
- DOI
- 10.4049/jimmunol.155.1.339
- PMID
- 7602110
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Language
- English
- Date published
- 07/01/1995
- Academic Unit
- Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Rheumatology, Allergy, and Immunology
- Record Identifier
- 9984065829802771
Metrics
23 Record Views