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Antigen inhibition of the interaction between murine monoclonal anti-idiotypic antibodies and human monoclonal anti-DNA antibodies
Journal article   Open access   Peer reviewed

Antigen inhibition of the interaction between murine monoclonal anti-idiotypic antibodies and human monoclonal anti-DNA antibodies

TAI Muryoi, TAKESHI Sasaki, EIICHI Tamate, OSAMU Takai, NAOKO Harata and KAORU Yoshinaga
Tohoku journal of experimental medicine, Vol.152(3), pp.253-258
1987
DOI: 10.1620/tjem.152.253
PMID: 3499013
url
https://doi.org/10.1620/tjem.152.253View
Published (Version of record) Open Access

Abstract

Monoclonal anti-idiotypic (Id) antibodies to human monoclonal anti-DNA antibodies were obtained by a somatic cell hybridization. One, termed as D1E2, was directed to Id of anti-single-stranded (ss) DNA antibody (0-81) and the other, 1F5, to anti-double-stranded (ds) DNA antibody (NE-1). Each anti-Id antibody behaved like a mirror image of the corresponding antigens, when determined by competitive inhibition radioimmunoassay. Therefore, D1E2 and 1F5 are regarded as Ab2β or Ab2γ. These antibodies will make useful reagents to understand and manipulate the autoantibody production in human.

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