Journal article
Antihyperalgesic effect of CB1 receptor activation involves the modulation of P2X(3) receptor in the primary afferent neuron
European journal of pharmacology, Vol.798, pp.113-121
03/05/2017
DOI: 10.1016/j.ejphar.2017.01.030
PMID: 28131783
Abstract
Cannabinoid system is a potential target for pain control. Cannabinoid receptor 1 (CB1) activation play a role in. the analgesic effect of cannabinoids once it is expressed in primary afferent neurons. This study investigates whether the anti-hyperalgesic effect of CB1 receptor activation involves P2X(3) receptor in primary afferent neurons. Mechanical hyperalgesia was evaluated by electronic von Frey test. Cannabinoid effect was evaluated using anandamide or ACEA, a non-selective or a selective CBI receptor agonists, respectively; AM251, a CB1 receptor antagonist, and antisense ODN for CB1. receptor. Calcium imaging assay was performed to evaluated alpha,beta-meATP-responsive cultured DRG neurons pretreated with ACEA. Anandamide or ACEA administered in peripheral tissue reduced the carrageenan-induced mechanical hyperalgesia. The reduction in the carrageenaninduced hyperalgesia induced by ACEA was completely reversed by administration of AM251 as well as by the intrathecal treatment with antisense ODN for CB1 receptor. Also, ACEA reduced the mechanical hyperalgesia induced by bradykinin and by alpha,beta-meATP, a P2X(3) receptor non-selective agonist, but not by tumor necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta) and chemokine-induced chemoattractant-1 (CINC-1). Finally, CBI receptors are co-localized with P2X(3) receptors in DRG small-diameter neurons and the treatment with ACEA reduced the number of alpha,beta-meATP-responsive cultured DRG neurons. Our data suggest that the analgesic effect of CB1. receptor activation is mediated by a negative modulation of the P2X(3) receptor in the primary afferent neurons.
Details
- Title: Subtitle
- Antihyperalgesic effect of CB1 receptor activation involves the modulation of P2X(3) receptor in the primary afferent neuron
- Creators
- Maria Claudia Goncalves Oliveira-Fusaro - Univ Estadual Campinas, Sch Appl Sci, BR-13484350 Sao Paulo, BrazilCristiane Isabel Silva Zanoni - Univ Ribeirao Preto, Fac Med, Dept Pharmacol, BR-14049900 Sao Paulo, BrazilGilson Goncalves dos Santos - Universidade Estadual de Campinas (UNICAMP)Luis Paulo Manzo - Universidade Estadual de Campinas (UNICAMP)Dioneia Araldi - Universidade Estadual de Campinas (UNICAMP)Ivan Jose Magayewski Bonet - Universidade Estadual de Campinas (UNICAMP)Claudia Herrera Tambeli - Universidade Estadual de Campinas (UNICAMP)Elayne Vieira Dias - Universidade Estadual de Campinas (UNICAMP)Carlos Amilcar Parada - Universidade Estadual de Campinas (UNICAMP)
- Resource Type
- Journal article
- Publication Details
- European journal of pharmacology, Vol.798, pp.113-121
- DOI
- 10.1016/j.ejphar.2017.01.030
- PMID
- 28131783
- ISSN
- 0014-2999
- eISSN
- 1879-0712
- Publisher
- Elsevier
- Number of pages
- 9
- Grant note
- CAPES (Coordination of Improvement of Higher Education Personnel), Brazil
- Language
- English
- Date published
- 03/05/2017
- Academic Unit
- Neuroscience and Pharmacology
- Record Identifier
- 9985177944702771
Metrics
1 Record Views