Journal article
Antimicrobial Efficacy Assessment of Human Derived Composite Amnion-Chorion Membrane
Scientific reports, Vol.9(1), pp.15600-11
10/30/2019
DOI: 10.1038/s41598-019-52150-4
PMCID: PMC6821917
PMID: 31666625
Abstract
Human derived composite amnion-chorion membrane (ACM) has been used to facilitate wound healing due to reported anti-inflammatory properties and promotion of cell proliferation. This study aimed to assess the antimicrobial properties of the ACM using novel methods to visualize the antimicrobial efficacy of membranes in situ at different time points. Porcine Pericardium Collagen Membranes (PPCM) served as membrane controls. Circular pieces of the membranes were used in three different assays: insert, agar contact and glass-bottom well assays. Streptococcus gordonii were spotted onto the membranes and the plates were subsequently centrifuged to ensure direct bacterial contact with the membranes in the insert and agar contact assays, thus better mimicking bacterial adherence in the oral cavity. After incubation at 37 °C for 8, 24, and 48 hours, the membranes were dyed with the Live/Dead BacLight Bacterial Viability fluorescence stain and analyzed via confocal microscopy. The results demonstrated that the ACM completely inhibited bacterial growth at all time points, whereas the PPCM did not demonstrate any antimicrobial properties. Within the limits of this study, the ACM showed extremely high antimicrobial efficacy against oral streptococci. In addition, our methods may be useful in assessing antimicrobial properties for biomaterials with minimum diffusion ability, when traditional assessment methods are not applicable.
Details
- Title: Subtitle
- Antimicrobial Efficacy Assessment of Human Derived Composite Amnion-Chorion Membrane
- Creators
- Nathan D Palanker - The University of Texas Health Science Center at HoustonChun-Teh Lee - The University of Texas Health Science Center at HoustonRobin L Weltman - The University of Texas Health Science Center at HoustonGena D Tribble - The University of Texas Health Science Center at HoustonRansome van der Hoeven - The University of Texas Health Science Center at HoustonJianming Hong - The University of Texas Health Science Center at HoustonBingyan Wang - The University of Texas Health Science Center at Houston
- Resource Type
- Journal article
- Publication Details
- Scientific reports, Vol.9(1), pp.15600-11
- DOI
- 10.1038/s41598-019-52150-4
- PMID
- 31666625
- PMCID
- PMC6821917
- NLM abbreviation
- Sci Rep
- ISSN
- 2045-2322
- eISSN
- 2045-2322
- Language
- English
- Date published
- 10/30/2019
- Academic Unit
- Dental Research; Periodontics
- Record Identifier
- 9984737966702771
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