Antimicrobial FiteBac® K21 promotes antimicrobial Potency and wound healing

Ranjeet Ajit Bapat; Rikan Libat; Ong Shu Yuin; Abhishek Parolia; Muhammad Sharjeel Ilyas; Abdul Samad Khan; Mak Kit Kay; Mallikarjuna Rao Pichika; Kirti Saxena; Liang Lin Seow; Preena Sidhu; Umer Daood

doi:10.1016/j.heliyon.2023.e19282

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Antimicrobial FiteBac® K21 promotes antimicrobial Potency and wound healing

Ranjeet Ajit Bapat, Rikan Libat, Ong Shu Yuin, Abhishek Parolia, Muhammad Sharjeel Ilyas, Abdul Samad Khan, Mak Kit Kay, Mallikarjuna Rao Pichika, Kirti Saxena, Liang Lin Seow, …

Heliyon, Vol.9(8), e19282

08/01/2023

DOI: 10.1016/j.heliyon.2023.e19282

PMCID: PMC10469996

PMID: 37664740

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Abstract

Objectives Successful root canal therapy is dependent on the efficacy of complete instrumentation and adequate use of chemical irrigant to eliminate the biofilm from dentin surface. The aim of the study was to examine antibiofilm and antimicrobial effectiveness of newly formulated Quaternary ammonium silane (QAS/also codenamed K21; against Fusobacterium nucleatum (F. nucleatum) and Enterococcus faecalis (E. faecalis) biofilm on radicular dentin with evaluation of the anti-inflammatory consequence in vivo. Methods Fourier Transform Infrared Spectroscopy (FTIR) was performed after complete hydrolysis of K21 solution. Human teeth were inoculated with biofilms for 7-days followed by treatment with various irrigants. The irrigant groups were Sodium hypochlorite [NaOCl (6%)], Chlorhexidine [CHX (2%)], K21 (0.5%), K21 (1%) and Saline. Scanning electron microscopy (SEM) was performed for biofilm and resin-dentin penetration. Transmission Electron Microscopy (TEM) of biofilms was done to evaluate application of K21. For in vivo evaluation, Albino wistar rats were injected subcutaneously and sections were stained with haematoxylin/eosin. Macrophage, M1/M2 expression were evaluated along with molecular simulation. Raman measurements were done on dried biofilms. Results FTIR K21 specimens demonstrated presence of ethanol/silanol groups. Raman band at 1359 cm−1 resemble to –CH2- wagging displaying 29Si atoms in Nuclear Magnetic Resonance (NMR). 0.5%K21 showed cells exhibiting folded membranes. SEM showed staggering amount of resin tags with 0.5% K21 group. TEM showed membrane disruption in K21-groups. K21 groups were initially irritant, which subsided completely afterwards showing increased CD68. K21 and MMP/collagen complex was thermodynamically favourable. Conclusion K21 root canal irrigant was able to penetrate bacterial wall and can serve as a potential irrigant for therapeutic benefits. Expression of M2 polarized subsets showed K21 can serve in resolving inflammation and potentiate tissue repair.

Details

Title: Subtitle: Antimicrobial FiteBac® K21 promotes antimicrobial Potency and wound healing
Creators: Ranjeet Ajit Bapat - IMU University
Rikan Libat - IMU University
Ong Shu Yuin - IMU University
Abhishek Parolia - IMU University
Muhammad Sharjeel Ilyas - Post Graduate Medical Institute
Abdul Samad Khan - Imam Abdulrahman Bin Faisal University
Mak Kit Kay - University of Kuala Lumpur
Mallikarjuna Rao Pichika - IMU University
Kirti Saxena - IMU University
Liang Lin Seow - IMU University
Preena Sidhu - IMU University
Umer Daood - IMU University
Resource Type: Journal article
Publication Details: Heliyon, Vol.9(8), e19282
DOI: 10.1016/j.heliyon.2023.e19282
PMID: 37664740
PMCID: PMC10469996
NLM abbreviation: Heliyon
ISSN: 2405-8440
eISSN: 2405-8440
Publisher: Elsevier
Grant note: DOI: 10.13039/501100009381, name: International Medical University
Language: English
Date published: 08/01/2023
Academic Unit: Endodontics
Record Identifier: 9984462983502771

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