Journal article
Antioxidant Diet Protects Against Emphysema, but Increases Mortality in Cigarette Smoke-Exposed Mice
Chronic obstructive pulmonary disease, Vol.8(5), pp.362-368
10/01/2011
DOI: 10.3109/15412555.2011.600361
PMID: 21834692
Abstract
Oxidative stress plays an important role in cigarette smoke-induced lung inflammation and emphysema. We produced an enriched diet by adding freeze-dried fruits and vegetables and additional supplements to the 8604 Teklad Rodent Diet, a standard rodent diet. In this study, we examined the effects of the antioxidant-enriched diet on cigarette smoke-induced lung inflammation and emphysema. CH3/HeN mice were fed either a regular diet or the supplemented diet. These mice were exposed to filtered air, a low concentration of cigarette smoke (total particulate matter: 100 mg/m3) or a high concentration of cigarette smoke (total particulate matter: 250 mg/m3) for 6 h/day, 5 days/week for total 16 weeks. Surprisingly, increased mortality (53%) was observed in the high concentration of cigarette smoke-exposed mice fed the antioxidant diet compared to the high concentration of cigarette smoke-exposed mice that were fed a regular diet (13%). The necropsy analysis revealed nasal passage obstruction due to mucous plugging in cigarette smoke-exposed mice on the antioxidant diet. However, the antioxidant diet significantly reduced neutrophilic inflammation and emphysema in the high concentration of cigarette smoke-exposed mice as compared to the regular diet /high concentration of cigarette smoke controls. The antioxidant capacity in the bronchoalveolar fluid or oxidative damage to the lung tissue was not affected by the antioxidant diet. Pro-MMP-2, MMP-2, and MMP-9 activity did not correlate with the protective effects of AOD on cigarette smoke-induced emphysema. These data suggest that the antioxidant diet reduced cigarette smoke-induced inflammation and emphysema, but increased mortality in the obligate nose-breathing mice.
Details
- Title: Subtitle
- Antioxidant Diet Protects Against Emphysema, but Increases Mortality in Cigarette Smoke-Exposed Mice
- Creators
- Toru Nyunoya - Lovelace Respiratory Research InstituteThomas H. March - Lovelace Respiratory Research InstituteYohannes Tesfaigzi - Lovelace Respiratory Research InstituteJeanClare Seagrave - Lovelace Respiratory Research Institute
- Resource Type
- Journal article
- Publication Details
- Chronic obstructive pulmonary disease, Vol.8(5), pp.362-368
- DOI
- 10.3109/15412555.2011.600361
- PMID
- 21834692
- ISSN
- 1541-2555
- eISSN
- 1541-2563
- Publisher
- Informa Healthcare
- Number of pages
- 7
- Language
- English
- Date published
- 10/01/2011
- Academic Unit
- Internal Medicine
- Record Identifier
- 9985214170302771
Metrics
1 Record Views