Journal article
Antioxidant Effects of N-Acetylcysteine Prevent Programmed Metabolic Disease in Mice
Diabetes (New York, N.Y.), Vol.69(8), pp.1650-1661
08/2020
DOI: 10.2337/db19-1129
PMCID: PMC7372077
PMID: 32444367
Abstract
An adverse maternal in utero and lactation environment can program offspring for increased risk for metabolic disease. The aim of this study was to determine whether N-acetylcysteine (NAC), an anti-inflammatory antioxidant, attenuates programmed susceptibility to obesity and insulin resistance in offspring of mothers on a high-fat diet (HFD) during pregnancy. CD1 female mice were acutely fed a standard breeding chow or HFD. NAC was added to the drinking water (1 g/kg) of the treatment cohorts from embryonic day 0.5 until the end of lactation. NAC treatment normalized HFD-induced maternal weight gain and oxidative stress, improved the maternal lipidome, and prevented maternal leptin resistance. These favorable changes in the in utero environment normalized postnatal growth, decreased white adipose tissue (WAT) and hepatic fat, improved glucose and insulin tolerance and antioxidant capacity, reduced leptin and insulin, and increased adiponectin in HFD offspring. The lifelong metabolic improvements in the offspring were accompanied by reductions in proinflammatory gene expression in liver and WAT and increased thermogenic gene expression in brown adipose tissue. These results, for the first time, provide a mechanistic rationale for how NAC can prevent the onset of metabolic disease in the offspring of mothers who consume a typical Western HFD.
Details
- Title: Subtitle
- Antioxidant Effects of N-Acetylcysteine Prevent Programmed Metabolic Disease in Mice
- Creators
- Maureen J Charron - Albert Einstein College of MedicineLyda Williams - Albert Einstein College of MedicineYoshinori Seki - Albert Einstein College of MedicineXiu Quan Du - Albert Einstein College of MedicineBhagirath Chaurasia - University of UtahAlan Saghatelian - Salk Institute for Biological StudiesScott A Summers - University of UtahEllen B Katz - Albert Einstein College of MedicinePatricia M Vuguin - Columbia UniversitySandra E Reznik - Albert Einstein College of Medicine
- Resource Type
- Journal article
- Publication Details
- Diabetes (New York, N.Y.), Vol.69(8), pp.1650-1661
- DOI
- 10.2337/db19-1129
- PMID
- 32444367
- PMCID
- PMC7372077
- ISSN
- 0012-1797
- eISSN
- 1939-327X
- Grant note
- F31 DK093332 / NIDDK NIH HHS R01 DK115824 / NIDDK NIH HHS R21 DK081194 / NIDDK NIH HHS R01 DK122001 / NIDDK NIH HHS
- Language
- English
- Date published
- 08/2020
- Academic Unit
- Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984359578002771
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