Journal article
Antioxidant and Antimutagenic Effect of Quercetin against DEN Induced Hepatotoxicity in Rat
Phytotherapy research, Vol.24(1), pp.119-128
01/01/2010
DOI: 10.1002/ptr.2883
PMID: 19504466
Abstract
Diethyinitrosamine (DEN), a potent hepatocarcinogen, is found in tobacco smoke, processed meat as well as in different food products. Quercetin (QC), a naturally occurring flavonoid has excellent antioxidant properties. The present study was aimed to investigate the chemoprotective potential of QC against DEN induced hepatotoxicity in Sprague-Dawley (SD) rats. Quercetin was administered (10, 30 and 100 mg/kg) for 5 consecutive days after DEN (200 mg/kg) treatment. The animals were killed 24 h after the last dose of QC/saline treatment. The DEN induced hepatotoxicity was evident by elevated malondialdehyde (MDA) and decreased glutathione (GSH) levels in the liver. A significant increase in the levels of plasma aspartate transaminase (AST) and plasma alanine transaminase (ALT) was observed in the DEN treated group. The DEN induced DNA damage was evaluated using a single cell gel electrophoresis (SCGE) assay. A significant increase in the number of TUNEL positive cells was observed in the DEN treated group. Quercetin restored AST, ALT and GSH levels at all the tested doses. Restoration of the MDA level and cellular morphology was observed at doses of 10 and 30 mg/kg of QC. Further, DEN induced DNA damage and apoptosis was ameliorated by QC. The results indicate that QC ameliorates the DEN induced hepatotoxicity in rats and can be a candidate for a good chemoprotectant. Copyright (C) 2009 John Wiley & Sons, Ltd.
Details
- Title: Subtitle
- Antioxidant and Antimutagenic Effect of Quercetin against DEN Induced Hepatotoxicity in Rat
- Creators
- C. Gupta - Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, S.A.S. Nagar, Mohali, Punjab-160 062, India.A. Vikram - Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, S.A.S. Nagar, Mohali, Punjab-160 062, India.D. N. Tripathi - Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, S.A.S. Nagar, Mohali, Punjab-160 062, India.P. Ramarao - Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Sector-67, S.A.S. Nagar, Mohali, Punjab-160 062, India.G. B. Jena - Department of Pharmacology and Toxicology, National Institute of Pharmaceutical Education and Research (NIPER), Sector‐67, S.A.S. Nagar, Mohali, Punjab‐160 062, India
- Resource Type
- Journal article
- Publication Details
- Phytotherapy research, Vol.24(1), pp.119-128
- Publisher
- Wiley
- DOI
- 10.1002/ptr.2883
- PMID
- 19504466
- ISSN
- 0951-418X
- eISSN
- 1099-1573
- Number of pages
- 10
- Grant note
- National Institute of Pharmaceutical Education and Research (NIPER) 111172/2K8/1 / CSIR; Council of Scientific & Industrial Research (CSIR) - India
- Language
- English
- Date published
- 01/01/2010
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984359927802771
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