Journal article
Antioxidant defense systems of two lipidopteran insect cell lines
Free radical biology & medicine, Vol.30(11), pp.1254-1262
06/2001
DOI: 10.1016/S0891-5849(01)00520-2
PMID: 11368923
Abstract
Spodoptera frugiperda Sf-9 (Sf-9) and Trichoplusia ni BTI-Tn-5B1-4 (Tn-5B1-4) insect cell lines were found to contain unique assemblages of antioxidant enzymes. Specifically, the Sf-9 insect cell line contained Manganese and Copper-Zinc superoxide dismutase (MnSOD and CuZnSOD) for reducing the superoxide radical (O(2)(*-)) to hydrogen peroxide (H(2)O(2)) and ascorbate peroxidase (APOX) for reducing the resulting H(2)O(2) to H(2)O. Approximately one third of the total SOD activity was found to be MnSOD. The Tn-5B1-4 cells were also found to contain MnSOD (approximately two thirds of the total SOD activity), CuZnSOD and APOX activities. However, the Tn-5B1-4 cell line, in contrast to the Sf-9 cell line, contained catalase (CAT) activity for reducing H(2)O(2) to H(2)O. Both the Sf-9 and Tn-5B1-4 cell lines contained glutathione reductase and dehydroascorbic acid reductase activities for regenerating the reduced forms of glutathione and ascorbic acid, respectively. In addition, both cell lines contained glutathione S-transferase peroxidase activity towards hydroperoxides other than H(2)O(2). Finally, neither cell line contains the glutathione peroxidase activity that is ubiquitous in mammalian cells.
Details
- Title: Subtitle
- Antioxidant defense systems of two lipidopteran insect cell lines
- Creators
- Ying WangLarry W OberleyDavid W Murhammer
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.30(11), pp.1254-1262
- DOI
- 10.1016/S0891-5849(01)00520-2
- PMID
- 11368923
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Language
- English
- Date published
- 06/2001
- Academic Unit
- Chemical and Biochemical Engineering
- Record Identifier
- 9984003421902771
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