Antioxidant proteins and reactive oxygen species are decreased in a murine epidermal side population with stem cell-like characteristics

Wanakee J Carr; Rebecca E Oberley-Deegan; Yuping Zhang; Christopher C Oberley; Larry W Oberley; Martine Dunnwald

doi:10.1007/s00418-011-0786-2

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Antioxidant proteins and reactive oxygen species are decreased in a murine epidermal side population with stem cell-like characteristics

Journal article

Peer reviewed

Antioxidant proteins and reactive oxygen species are decreased in a murine epidermal side population with stem cell-like characteristics

Wanakee J Carr, Rebecca E Oberley-Deegan, Yuping Zhang, Christopher C Oberley, Larry W Oberley and Martine Dunnwald

Histochemistry and cell biology, Vol.135(3), pp.293-304

03/2011

DOI: 10.1007/s00418-011-0786-2

PMCID: PMC3401960

PMID: 21347610

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Abstract

Reactive oxygen species (ROS) and antioxidants are essential to maintain a redox balance within tissues and cells. Intracellular ROS regulate key cellular functions such as proliferation, differentiation and apoptosis through cellular signaling, and response to injury. The redox environment is particularly important for stem/progenitor cells, as their self-renewal and differentiation has been shown to be redox sensitive. However, not much is known about ROS and antioxidant protein function in freshly isolated keratinocytes, notably the different keratinocyte subpopulations. Immunostaining of neonatal cutaneous sections revealed that antioxidant enzymes [catalase, SOD2, gluthatione peroxidase-1 (GPx)] and ROS are localized predominantly to the epidermis. We isolated keratinocyte subpopulations and found lower levels of SOD2, catalase and GPx, as well as decreased SOD and catalase activity in an epidermal side population with stem cell-like characteristics (EpSPs) compared to more differentiated (Non-SP) keratinocytes. EpSPs also exhibited less mitochondrial area, fewer peroxisomes and produced lower levels of ROS than Non-SPs. Finally, EpSPs were more resistant to UV radiation than their progeny. Together, our data indicate ROS and antioxidant levels are decreased in stem-like EpSPs.

Keratinocytes

Oxidative stress

Reactive oxygen species

Mouse

Anti-oxidant protein

Stem cells

Details

Title: Subtitle: Antioxidant proteins and reactive oxygen species are decreased in a murine epidermal side population with stem cell-like characteristics
Creators: Wanakee J Carr - Department of Pediatrics, 206 MRC, The University of Iowa, Iowa City, IA 52242, USA
Rebecca E Oberley-Deegan - Department of Medicine, National Jewish Health, Denver, CO 80206, USA
Yuping Zhang - Department of Radiation Oncology, The University of Iowa, Iowa City, IA 52242, USA. Department of Free Radical Biology Program, The University of Iowa, Iowa City, IA 52242, USA
Christopher C Oberley - Department of Pediatrics, 206 MRC, The University of Iowa, Iowa City, IA 52242, USA
Larry W Oberley - Department of Radiation Oncology, The University of Iowa, Iowa City, IA 52242, USA. Department of Free Radical Biology Program, The University of Iowa, Iowa City, IA 52242, USA
Martine Dunnwald - Department of Pediatrics, 206 MRC, The University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Histochemistry and cell biology, Vol.135(3), pp.293-304
DOI: 10.1007/s00418-011-0786-2
PMID: 21347610
PMCID: PMC3401960
NLM abbreviation: Histochem Cell Biol
ISSN: 0948-6143
eISSN: 1432-119X
Grant note: R03 AR055313-01A1 || AR / National Institute of Arthritis and Musculoskeletal and Skin Diseases : NIAMS
Language: English
Date published: 03/2011
Academic Unit: Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Obstetrics and Gynecology
Record Identifier: 9984025463902771

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