Journal article
Antioxidants attenuate myocyte apoptosis in the remote non-infarcted myocardium following large myocardial infarction
Cardiovascular research, Vol.45(3), pp.679-687
02/2000
DOI: 10.1016/S0008-6363(99)00400-9
PMID: 10728389
Abstract
Increased oxidative stress and myocyte apoptosis co-exist in the remote non-infarcted myocardium (RM) following a large myocardial infarction. We proposed that these phenomena are causally related.
On day 3 after induction of myocardial infarction, Sprague-Dawley rats were randomized to receive probucol and pyrrolidine dithiocarbamate (MI-T), or vehicle only (MI) for 7 weeks. Control rats (C) received vehicle. At 7 weeks, lipidperoxidation within the RM was assessed by measuring thiobarbituric acid reactive substances, which were significantly increased in MI vs. C, while MI-T was not different from C. There was a significant increase in cardiac myocytes positive for in situ TdT-UTP nick-end labeling within the RM in MI vs. C, which was inhibited in MI-T. Furthermore, internucleosomal DNA fragmentation was clearly demonstrated on agarose gels from RM in the MI group, while it was much less apparent on gels from RM in the C and MI-T groups. Western blot analysis showed a significant increase in p53, Bax and caspase-3 protein expression within the RM of MI vs. C, all of which were inhibited in the MI-T group. Furthermore, there was evidence for an increase in caspase-3 activity within the RM from MI vs. C, which was normalized in the MI-T group.
Long-term treatment with the antioxidants probucol and pyrrolidine dithiocarbamate attenuates oxidative stress, myocyte apoptosis, caspase-3 like activity and the expression of p53, bax and caspase-3 within RM in rats after a large myocardial infarction.
Details
- Title: Subtitle
- Antioxidants attenuate myocyte apoptosis in the remote non-infarcted myocardium following large myocardial infarction
- Creators
- Helgi J Oskarsson - Department of Internal Medicine, University of Iowa, Iowa City. hoskarsson@hprhs.comLawrence CoppeyRobert M WeissWei-Gen Li
- Resource Type
- Journal article
- Publication Details
- Cardiovascular research, Vol.45(3), pp.679-687
- DOI
- 10.1016/S0008-6363(99)00400-9
- PMID
- 10728389
- ISSN
- 0008-6363
- eISSN
- 1755-3245
- Grant note
- DK25295 / NIDDK NIH HHS
- Language
- English
- Date published
- 02/2000
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984094670002771
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