Journal article
Antiviral activity of WIN 54954 in coxsackievirus B2 carrier state infected human myocardial fibroblasts
Antiviral research, Vol.37(1), pp.47-56
1998
DOI: 10.1016/S0166-3542(97)00056-9
PMID: 9497072
Abstract
Persistent infections with a cardiotropic enterovirus, e.g. coxsackievirus B2 (CVB2), cause chronic myocarditis and eventually congestive heart failure. Therefore, the antiviral activity of WIN 54954, a capsid binding antiviral agent that inhibits enterovirus uncoating, was studied in persistently CVB2-infected cultures of human myocardial fibroblasts. Cultures displayed a typical carrier state infection with virus titers of 3.9±1.6×10
5 plaque forming units (PFU)/ml and 0.99% infected cells. WIN 54954 (0.025–1
μg/ml) application was started 7 days after infection of the cultures. Compared to the WIN 54954 concentration resulting in a 90% plaque number reduction (EC
90=0.197
μg/ml) in acutely infected Vero cells, WIN 54954 reduced virus yields of myocardial fibroblast cultures more efficiently, e.g. more than 100 fold (99%) with 0.025
μg/ml after 4 days of application. Antiviral effects of WIN 54954 increased with application time and at 0.025
μg/ml Win 54954 completely inhibited infectious virus progeny after 16 days. Increasing the WIN 54954 concentration up to 1
μg/ml did not cause a greater inhibition of virus replication. In situ hybridization demonstrated that at 0.1
μg/ml WIN 54954 reduced the number of infected cells from 0.99 to 0.18%, although a complete eradication of CVB2-infected cells was not achieved at concentrations as high as 1
μg/ml. In conclusion, the results indicate that low concentrations of WIN 54954 are effective in treating persistent enterovirus infections of myocardial fibroblasts, although a complete eradication of the infection is not achieved with WIN 54954 as a single antiviral agent.
Details
- Title: Subtitle
- Antiviral activity of WIN 54954 in coxsackievirus B2 carrier state infected human myocardial fibroblasts
- Creators
- Albert Heim - Institut für Virologie und Seuchenhygiene, Medizinische Hochschule Hannover, Carl-Neuberg Str. 1, D-30625 Hannover, GermanyUte Pfetzing - Institut für Virologie und Seuchenhygiene, Medizinische Hochschule Hannover, Carl-Neuberg Str. 1, D-30625 Hannover, GermanyGudrun Müller - Abteilung für Kardiologie, Georg-August-University, Göttingen, GermanyIsabella Maria Grumbach - Abteilung für Kardiologie, Georg-August-University, Göttingen, Germany
- Resource Type
- Journal article
- Publication Details
- Antiviral research, Vol.37(1), pp.47-56
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0166-3542(97)00056-9
- PMID
- 9497072
- ISSN
- 0166-3542
- eISSN
- 1872-9096
- Language
- English
- Date published
- 1998
- Academic Unit
- Cardiovascular Medicine; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984094639702771
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