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Análisis mutacional del gen Homeobox de segmento muscular 1 (MSX1) en chilenos con fisuras orales
Journal article   Open access   Peer reviewed

Análisis mutacional del gen Homeobox de segmento muscular 1 (MSX1) en chilenos con fisuras orales

Alexandre R Vieira, Silvia Castillo Taucher, Teresa Aravena, Carmen Astete, Patricia Sanz, María Eugenia Tastets, Luis Monasterio and Jeffrey C Murray
Revista medíca de Chile, Vol.132(7), pp.816-822
07/01/2004
DOI: 10.4067/S0034-98872004000700005
PMID: 15379328
url
https://doi.org/10.4067/S0034-98872004000700005View
Published (Version of record) Open Access

Abstract

Background: Mutations of the MSX1 gene may contribute to nonsyndromic forms of cleft lip and/or cleft palate. Aim: To search for mutations of MSX1 coding regions, including one highly conserved non-coding region in the single intron, among Chilean patients with cleft lip/palate. Patients and Methods: We studied 45 patients with cleft lip/palate and their parents. Oral mucosa samples were obtained with a swab. DNA was extracted and amplified by PCR. Results: Two missense mutations (G16D and G34A) were identified in this study that may be useful for future admixture studies. The G16D mutation appears to disrupt a possible splicing site and may contribute to clefting in this population. Conclusions: Rare MSX1 mutations are found in some cases of cleft lip and/or cleft palate but others remain to be found most likely in other regulatory regions of the gene
MEDICINE, GENERAL & INTERNAL

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