Journal article
Aphidicolin-Induced FRA3B Breakpoints Cluster in Two Distinct Regions
Genomics (San Diego, Calif.), Vol.41(3), pp.485-488
05/01/1997
DOI: 10.1006/geno.1997.4690
PMID: 9169152
Abstract
The common fragile site at chromosomal band 3p14.2 (FRA3B) is the most sensitive single site in the human genome to induced chromosomal lesions. This fragile site may predispose chromosome 3p to breakage that is commonly observed in lung, renal, and many other cancers. We previously used aphidicolin induction of FRA3B expression in a chromosome 3-only somatic cell hybrid to generate a series of hybrids with breakpoints in the 3p14.2 region. These breakpoints were localized to two distinct clusters, separated by 200 kb, that lie on either side of a region of frequent breakage within FRA3B as observed by FISH analysis. Seven proximal aphidicolin-induced breakpoints were localized at or near the end of a THE element. The THE-1 element is flanked by LINE andAlurepetitive elements. The eight distal aphidicolin-induced breakpoints clustered in a region capable of forming multiple hairpin-like structures. Thus repetitive elements and hairpin-like structures may be responsible for chromosome fragility in this region.
Details
- Title: Subtitle
- Aphidicolin-Induced FRA3B Breakpoints Cluster in Two Distinct Regions
- Creators
- Liang Wang - Mayo ClinicWilliam Paradee - Emory UniversityChadwick Mullins - Wayne State UniversityRavi Shridhar - The Barbara Ann Karmanos Cancer InstituteRita Rosati - The Barbara Ann Karmanos Cancer InstituteCharles M. Wilke - University of Michigan Medical SchoolThomas W. Glover - University of Michigan–Ann ArborDavid I. Smith - Division of Experimental Pathology, Department of Laboratory Medicine and Pathology, Mayo Foundation, Rochester, Minnesota, 55905
- Resource Type
- Journal article
- Publication Details
- Genomics (San Diego, Calif.), Vol.41(3), pp.485-488
- Publisher
- Elsevier Inc
- DOI
- 10.1006/geno.1997.4690
- PMID
- 9169152
- ISSN
- 0888-7543
- eISSN
- 1089-8646
- Language
- English
- Date published
- 05/01/1997
- Academic Unit
- Medicine Administration
- Record Identifier
- 9984622050002771
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