Apolipoprotein M promoter polymorphisms alter promoter activity and confer the susceptibility to the development of type 1 diabetes

Xiaopan Wu; Nifang Niu; Kerstin Brismar; Xilin Zhu; Xin Wang; Suad Efendic; Te Du; Yang Liu; Harvest F Gu; Ying Liu

doi:10.1016/j.clinbiochem.2008.10.008

Back

Apolipoprotein M promoter polymorphisms alter promoter activity and confer the susceptibility to the development of type 1 diabetes

Journal article

Peer reviewed

Apolipoprotein M promoter polymorphisms alter promoter activity and confer the susceptibility to the development of type 1 diabetes

Xiaopan Wu, Nifang Niu, Kerstin Brismar, Xilin Zhu, Xin Wang, Suad Efendic, Te Du, Yang Liu, Harvest F Gu and Ying Liu

Clinical biochemistry, Vol.42(1-2), pp.17-21

01/2009

DOI: 10.1016/j.clinbiochem.2008.10.008

PMID: 19007767

View Online

Abstract

Apolipoprotein M plays an important role in the formation of prebeta-HDL and cholesterol efflux to HDL. In the present study, we investigate the potential association between the ApoM promoter polymorphisms and type 1 diabetes. The study was conducted in Peking Union Medical College, Beijing, China and Karolinska Institutet, Stockholm, Sweden. Two populations, including 493 Han Chinese subjects (177 T1D patients/316 controls) and 225 Swedish (124/101), are enrolled in the present study. Three single nucleotide polymorphisms (SNP) C-1065A, T-855C and T-778C in the promoter region of the ApoM gene are genotyped using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol. Promoter activity was measured by reporter gene assay. SNP T-778C was strongly associated with T1D in both Han Chinese (p=0.002, OR=2.188, CI 95%=1.338-3.581) and Swedish (p=0.021, OR=2.865, CI 95%=1.128-7.278) populations. The luciferase activity of -778C promoter was 1.41 times as high as that of -778T promoter (9.90+/-1.92 vs. 7.04+/-0.76, p=0.001). Allele C of SNP T-778C may increase promoter activity and confer the risk susceptibility to the development of T1D.

China

Polymerase Chain Reaction

Apolipoproteins - genetics

Apolipoproteins - physiology

Apolipoproteins M

Asian Continental Ancestry Group

Cell Line

Diabetes Mellitus, Type 1 - epidemiology

Diabetes Mellitus, Type 1 - genetics

European Continental Ancestry Group

Genetic Predisposition to Disease

Genotype

Humans

Lipocalins

Polymorphism, Restriction Fragment Length - genetics

Polymorphism, Single Nucleotide - genetics

Polymorphism, Single Nucleotide - physiology

Promoter Regions, Genetic - genetics

Details

Title: Subtitle: Apolipoprotein M promoter polymorphisms alter promoter activity and confer the susceptibility to the development of type 1 diabetes
Creators: Xiaopan Wu - Chinese Academy of Medical Sciences & Peking Union Medical College
Nifang Niu
Kerstin Brismar
Xilin Zhu
Xin Wang
Suad Efendic
Te Du
Yang Liu
Harvest F Gu
Ying Liu
Resource Type: Journal article
Publication Details: Clinical biochemistry, Vol.42(1-2), pp.17-21
DOI: 10.1016/j.clinbiochem.2008.10.008
PMID: 19007767
ISSN: 0009-9120
eISSN: 1873-2933
Language: English
Date published: 01/2009
Academic Unit: Stead Family Department of Pediatrics; Medical Genetics and Genomics
Record Identifier: 9984701557202771

Metrics

15 Record Views

31 Times Cited - Web of Science