Journal article
Appetitive Learning Requires the Alpha1-Like Octopamine Receptor OAMB in the Drosophila Mushroom Body Neurons
The Journal of neuroscience, Vol.33(4), pp.1672-1677
01/23/2013
DOI: 10.1523/JNEUROSCI.3042-12.2013
PMCID: PMC5634613
PMID: 23345239
Abstract
Associative learning is a fundamental form of behavioral plasticity. Octopamine plays central roles in various learning types in invertebrates; however, the target receptors and underlying mechanisms are poorly understood.
Drosophila
provides a powerful system to uncover the mechanisms for learning and memory. Here, we report that OAMB in the mushroom body neurons mediates the octopamine's signal for appetitive olfactory learning. The octopamine receptor OAMB has two isoforms (OAMB-K3 and OAMB-AS), differing in the third cytoplasmic loop and downstream sequence. The activation of each OAMB isoform increases intracellular Ca
2+
similar to the alpha1 adrenergic receptor, while OAMB-K3 additionally stimulates cAMP production. The
oamb
-null mutants showed severely impaired learning in appetitive olfactory conditioning that tests flies' capacity to learn and remember the odor associated with sugar reward. This deficit was also seen in the hypomorphic mutant with reduced OAMB expression in the mushroom bodies, the brain structure crucial for olfactory conditioning. Consistently, the
oamb
mutant's learning phenotype was fully rescued by conditional expression of either OAMB isoform in the mushroom body αβ and γ neurons. These results indicate that the OAMB receptor is a key molecule mediating the octopamine's signal for appetitive olfactory learning and its functional site is the mushroom body αβ and γ neurons. This study represents a critical step forward in understanding the cellular mechanism and neural circuit mediating reward learning and memory.
Details
- Title: Subtitle
- Appetitive Learning Requires the Alpha1-Like Octopamine Receptor OAMB in the Drosophila Mushroom Body Neurons
- Creators
- Young-Cho Kim - Department of Biological Sciences, Border Biomedical Research Center Neuroscience and Metabolic Disorders Project, University of Texas at El Paso, El Paso, Texas 79968Hyun-Gwan Lee - Department of Biological Sciences, Border Biomedical Research Center Neuroscience and Metabolic Disorders Project, University of Texas at El Paso, El Paso, Texas 79968Junghwa Lim - Department of Biological Sciences, Border Biomedical Research Center Neuroscience and Metabolic Disorders Project, University of Texas at El Paso, El Paso, Texas 79968Kyung-An Han - Department of Biological Sciences, Border Biomedical Research Center Neuroscience and Metabolic Disorders Project, University of Texas at El Paso, El Paso, Texas 79968
- Resource Type
- Journal article
- Publication Details
- The Journal of neuroscience, Vol.33(4), pp.1672-1677
- DOI
- 10.1523/JNEUROSCI.3042-12.2013
- PMID
- 23345239
- PMCID
- PMC5634613
- NLM abbreviation
- J Neurosci
- ISSN
- 0270-6474
- eISSN
- 1529-2401
- Publisher
- Society for Neuroscience
- Language
- English
- Date published
- 01/23/2013
- Academic Unit
- Neurology; Iowa Neuroscience Institute
- Record Identifier
- 9984020788102771
Metrics
20 Record Views