Application of lyophilized gene-delivery formulations to dental implant surfaces: non-cariogenic lyoprotectant preserves transfection activity of polyplexes long-term

Walla I Malkawi; Noah Z Laird; Pornpoj Phruttiwanichakun; Esraa Mohamed; Satheesh Elangovan; Aliasger K Salem

doi:10.1016/j.xphs.2022.11.008

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Application of lyophilized gene-delivery formulations to dental implant surfaces: non-cariogenic lyoprotectant preserves transfection activity of polyplexes long-term

Journal article

Peer reviewed

Application of lyophilized gene-delivery formulations to dental implant surfaces: non-cariogenic lyoprotectant preserves transfection activity of polyplexes long-term

Walla I Malkawi, Noah Z Laird, Pornpoj Phruttiwanichakun, Esraa Mohamed, Satheesh Elangovan and Aliasger K Salem

Journal of pharmaceutical science, Vol.112(1), pp.83-90

01/2023

DOI: 10.1016/j.xphs.2022.11.008

PMCID: PMC9772140

PMID: 36372226

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https://www.ncbi.nlm.nih.gov/pmc/articles/9772140View

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Abstract

Titanium is the metal of choice for dental implants because of its biocompatibility and ability to merge with human bone tissue. Despite the great success rate of dental implants, early and late complications occur. Coating titanium dental implant surfaces with polyethyleneimine (PEI)-plasmid DNA (pDNA) polyplexes improve osseointegration by generating therapeutic protein expression at the implantation site. Lyophilization is an approach for stabilizing polyplexes and extending their shelf life; however, most lyoprotectants are sugars that can aid bacterial growth in the peri-implant environment. In our research, we coated titanium surfaces with polyplex solutions containing varying amounts of lyoprotectants. We used two common lyoprotectants (sucrose and polyvinylpyrrolidone K30) and showed for the first time that sucralose (a sucrose derivative used as an artificial sweetener) might act as a lyoprotectant for polyplex solutions. Human embryonic kidney (HEK) 293T cells were used to quantify the transfection efficiency and cytotoxicity of the polyplex/lyoprotectant formulations coating titanium surfaces. Polyplexes that were lyophilized in the presence of a lyoprotectant displayed both preserved particle size and high transfection efficiencies. Polyplexes lyophilized in 2% sucralose have maintained transfection efficacy for three years. These findings suggest that modifying dental implants with lyophilized polyplexes might improve their success rate in the clinic.

Details

Title: Subtitle: Application of lyophilized gene-delivery formulations to dental implant surfaces: non-cariogenic lyoprotectant preserves transfection activity of polyplexes long-term
Creators: Walla I Malkawi - University of Iowa
Noah Z Laird - University of Iowa
Pornpoj Phruttiwanichakun - University of Iowa
Esraa Mohamed - University of Iowa
Satheesh Elangovan - University of Iowa
Aliasger K Salem - University of Iowa
Resource Type: Journal article
Publication Details: Journal of pharmaceutical science, Vol.112(1), pp.83-90
DOI: 10.1016/j.xphs.2022.11.008
PMID: 36372226
PMCID: PMC9772140
NLM abbreviation: J Pharm Sci
ISSN: 0022-3549
eISSN: 1520-6017
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: P30 CA086862, R21 DE031042-01A1
Language: English
Electronic publication date: 11/10/2022
Date published: 01/2023
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Periodontics
Record Identifier: 9984317460002771

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