Journal article
Application of near-infrared spectra on temperature-controlled protein crystallization a simulation study
Applied biochemistry and biotechnology, Vol.94(2), pp.179-196
2001
DOI: 10.1385/ABAB:94:2:179
PMID: 11456296
Abstract
Large, high-quality protein crystals are required for the structural determination of proteins via X-ray diffraction. In this article, we propose a technique to facilitate the production of such crystals and validate its feasibility through simulations. An analytical method for protein aqueous solution based on a Fourier transform infrared (FTIR) spectroscopy is combined with a temperature control strategy to manipulate the extent of supersaturation during crystal growth, thus impacting crystal quality. The technique requires minimal knowledge about the growth kinetics a priori. The simulations show that, under ideal conditions, the design can be as effective as predesigned temperature programs for crystallization based on known growth kinetics. Two kinds of errors might be encountered with this design. Error in the estimated number of seed crystals can result in a growth rate deviating from the desired one. Nevertheless, the deviation is usually tolerable and system instability is unlikely to occur. Based on the standard error of our FTIR method, errors in concentration measurement are simulated. Measurement error can result in system instability and impair the control algorithm. Such errors may be compensated by limiting the temperature change taken by the control action, or by improving the measurement precision through the use of regressed concentrations. Through simulations, it is shown that the proposed design is practical and represents a significant improvement over the commonly used isothermal crystallization technique.
Details
- Title: Subtitle
- Application of near-infrared spectra on temperature-controlled protein crystallization a simulation study
- Creators
- Shih-Yao B HU - Department of Chemical and Biochemical Engineering and Center of Optical Science and Technology, University of Iowa, 280 IA TL, Iowa City, IA 52242, United StatesJohn M Wiencek - Department of Chemical and Biochemical Engineering and Center of Optical Science and Technology, University of Iowa, 280 IA TL, Iowa City, IA 52242, United StatesMark A Arnold - Department of Chemistry and Center of Optical Science and Technology, University of Iowa, 280 IA TL, Iowa City, IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Applied biochemistry and biotechnology, Vol.94(2), pp.179-196
- Publisher
- Springer
- DOI
- 10.1385/ABAB:94:2:179
- PMID
- 11456296
- ISSN
- 0273-2289
- eISSN
- 1559-0291
- Language
- English
- Date published
- 2001
- Academic Unit
- Center for Biocatalysis and Bioprocessing; Fraternal Order of Eagles Diabetes Research Center; Chemistry
- Record Identifier
- 9984216721002771
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