Journal article
Application of restriction endonuclease analysis of chromosomal DNA in the study of Staphylococcus aureus colonization in continuous ambulatory peritoneal dialysis patients
Diagnostic microbiology and infectious disease, Vol.15(3), pp.195-199
1992
DOI: 10.1016/0732-8893(92)90113-8
PMID: 1582163
Abstract
The relationship between nasal and skin colonization with Staphylococcus aureus
and subsequent infection in continuous ambulatory peritoneal dialysis (CAPD) patients in Brazil has been documented by restriction endonuclease analysis of plasmid DNA. However, S. aureus
strains without detectable plasmids have been identified. Using restriction endonuclease analysis of chromosomal DNA hybridized with an rRNA gene probe, we document the diversity of S. aureus
strains without detectable plasmids colonizing CAPD patients. Ten paired strains without detectable plasmids from five patients were studied by restriction endonuclease analysis of chromosomal DNA and by phage typing. Five different profiles were obtained by restriction endonuclease analysis of chromosomal DNA. Although four of the ten paired isolates were nontypeable by phage typing, all were discriminated by restriction endonuclease analysis of chromosomal DNA. These results demonstrate that restriction endonuclease analysis of chromosomal DNA is a useful epidemiologic tool and complements the restriction endonuclease analysis of plasmid DNA of S. aureus
by providing a means of typing strains without detectable plasmids.
Details
- Title: Subtitle
- Application of restriction endonuclease analysis of chromosomal DNA in the study of Staphylococcus aureus colonization in continuous ambulatory peritoneal dialysis patients
- Creators
- Antonio PignatariLinda D BoykenLoreen A HerwaldtRichard HollisIvani LemeRicardo SessoMichael A Pfaller
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.15(3), pp.195-199
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0732-8893(92)90113-8
- PMID
- 1582163
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Language
- English
- Date published
- 1992
- Academic Unit
- Infectious Diseases; Epidemiology; Pathology; Internal Medicine
- Record Identifier
- 9984094747902771
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