Journal article
Application of staphylococcal enterotoxin B on normal and atopic skin induces up-regulation of T cells by a superantigen-mediated mechanism
Journal of allergy and clinical immunology, Vol.105(4), pp.820-826
04/2000
DOI: 10.1067/mai.2000.105524
PMID: 10756235
Abstract
Background: The skin of patients with inflammatory skin diseases such as atopic dermatitis is frequently colonized with Staphylococcus aureus. Colonization with S aureus has been reported to exacerbate atopic dermatitis. Recent studies have demonstrated that S aureus isolated from the skin of patients with atopic dermatitis releases bacterial toxins that act as superantigens. We have previously applied the staphylococcal superantigen staphylococcal enterotoxin B (SEB) on intact human skin and found that the application led to induction of dermatitis. Objective: The purpose of the study was to determine whether superantigen-induced dermatitis is primarily due to a T cell–superantigen–mediated reaction or represents nonspecific cytokine-driven inflammation. Methods: We applied SEB, vehicle, and sodium lauryl sulfate on normal skin in healthy (n = 6) and atopic subjects (n = 6) and biopsy specimens were taken from all treated areas. The biopsy specimens from all subjects and peripheral blood from the atopic subjects were analyzed for the T-cell receptor (TCR) Vβ repertoire with mAbs against TCR Vβ 2, 3, 8.1, 12, 14, and 17. Results: From all subjects, both healthy and patients with atopic dermatitis, skin biopsy specimens from SEB-treated areas demonstrated selective accumulation of T cells expressing SEB-reactive TCR Vβ 12 and 17 (P < .05). This selective up-regulation was not found in the sodium lauryl sulfate–treated areas. Conclusion: Our data strongly support that superantigen-induced T-cell activation is involved in the dermatitis seen after experimental application of SEB on intact skin. (J Allergy Clin Immunol 2000;105:820-6.)
Details
- Title: Subtitle
- Application of staphylococcal enterotoxin B on normal and atopic skin induces up-regulation of T cells by a superantigen-mediated mechanism
- Creators
- Lone SkovJeanett V OlsenRalph GiornoPatrick M SchlievertOle BaadsgaardDonald Y.M Leung
- Resource Type
- Journal article
- Publication Details
- Journal of allergy and clinical immunology, Vol.105(4), pp.820-826
- DOI
- 10.1067/mai.2000.105524
- PMID
- 10756235
- NLM abbreviation
- J Allergy Clin Immunol
- ISSN
- 0091-6749
- eISSN
- 1097-6825
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 04/2000
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001207202771
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