Applying gene silencing technology to contraception

Gregory A Dissen; Alejandro Lomniczi; Ryan L Boudreau; Yong Hong Chen; Beverly L Davidson; Sergio R Ojeda

doi:10.1111/rda.12016

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Applying gene silencing technology to contraception

Journal article

Open access

Applying gene silencing technology to contraception

Gregory A Dissen, Alejandro Lomniczi, Ryan L Boudreau, Yong Hong Chen, Beverly L Davidson and Sergio R Ojeda

Reproduction in domestic animals, Vol.47(Suppl 6), pp.381-386

12/2012

DOI: 10.1111/rda.12016

PMCID: PMC3598614

PMID: 23279544

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Abstract

Population control of feral animals is often difficult, as it can be dangerous for the animals, labor intensive, and expensive. Therefore, a useful tool for control of animal populations would be a nonsurgical method to induce sterility. Our laboratories utilize methods aimed at targeting brain cells\nin vivo\nwith vehicles that deliver a payload of either inhibitory RNAs or genes intended to correct cellular dysfunction. A useful framework for design of a new approach will be the combination of these methods with the intended goal to produce a technique that can be used to noninvasively sterilize cats and dogs. For this approach to succeed it has to meet several conditions: The target gene must be essential for fertility; the method must include a mechanism to effectively and specifically silence the gene of interest; the method of delivering the silencing agent must be minimally invasive, and finally, the silencing effect must be sustained for the lifespan of the target species, so that expansion of the population can be effectively prevented. In this article we discuss our work to develop gene silencing technology to induce sterility; we will use examples of our previous studies demonstrating that this approach is viable. These studies include: a) the use of viral vectors able to disrupt reproductive cyclicity when delivered to the regions of the brain involved in the control of reproduction, and b) experiments with viral vectors that are able to ameliorate neuronal disease when delivered systemically using a novel approach of gene therapy.

microRNA

Adeno-associated virus

RNA interference

Details

Title: Subtitle: Applying gene silencing technology to contraception
Creators: Gregory A Dissen - Division of Neuroscience, Oregon National Primate Research Center-Oregon Health & Science University, Beaverton, OR, USA
Alejandro Lomniczi - Division of Neuroscience, Oregon National Primate Research Center-Oregon Health & Science University, Beaverton, OR, USA
Ryan L Boudreau - Department of Internal Medicine, University of Iowa, Iowa City, USA
Yong Hong Chen - Department of Internal Medicine, University of Iowa, Iowa City, USA
Beverly L Davidson - Department of Internal Medicine, University of Iowa, Iowa City, USA
Sergio R Ojeda - Division of Neuroscience, Oregon National Primate Research Center-Oregon Health & Science University, Beaverton, OR, USA
Resource Type: Journal article
Publication Details: Reproduction in domestic animals, Vol.47(Suppl 6), pp.381-386
DOI: 10.1111/rda.12016
PMID: 23279544
PMCID: PMC3598614
NLM abbreviation: Reprod Domest Anim
ISSN: 0936-6768
eISSN: 1439-0531
Grant note: P51 RR000163 || RR / National Center for Research Resources : NCRR\nP51 OD011092 || OD / Office of the Director : NIH
Language: English
Date published: 12/2012
Academic Unit: Iowa Neuroscience Institute; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
Record Identifier: 9984070721602771

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