Journal article
Approaches to Evaluate Lung Inflammation in Translational Research
Veterinary pathology, Vol.55(1), pp.42-52
01/2018
DOI: 10.1177/0300985817726117
PMCID: PMC5600706
PMID: 28812529
Abstract
Inflammation is a common feature in several types of lung disease and is a frequent end point to validate lung disease models, evaluate genetic or environmental impact on disease severity, or test the efficacy of new therapies. Questions relevant to a study should be defined during experimental design and techniques selected to specifically address these scientific queries. In this review, the authors focus primarily on the breadth of techniques to evaluate lung inflammation that have both clinical and preclinical applications. Stratification of approaches to assess lung inflammation can diminish weaknesses inherent to each technique, provide data validation, and increase the reproducibility of a study. Specialized techniques (eg, imaging, pathology) often require experienced personnel to collect, evaluate, and interpret the data; these experts should be active contributors to the research team through reporting of the data. Scoring of tissue lesions is a useful method to transform observational pathologic data into semiquantitative or quantitative data for statistical analysis and enhanced rigor. Each technique to evaluate lung inflammation has advantages and limitations; understanding these parameters can help identify approaches that best complement one another to increase the rigor and translational significance of data.
Details
- Title: Subtitle
- Approaches to Evaluate Lung Inflammation in Translational Research
- Creators
- David K Meyerholz - 1 Department of Pathology, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA, USAJessica C Sieren - 3 Department of Biomedical Engineering, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA, USAAmanda P Beck - 4 Department of Pathology, Albert Einstein College of Medicine, Bronx, NY, USAHeather A Flaherty - 5 Department of Veterinary Pathology, Iowa State University, Ames, IA, USA
- Resource Type
- Journal article
- Publication Details
- Veterinary pathology, Vol.55(1), pp.42-52
- Publisher
- United States
- DOI
- 10.1177/0300985817726117
- PMID
- 28812529
- PMCID
- PMC5600706
- ISSN
- 0300-9858
- eISSN
- 1544-2217
- Grant note
- P30 ES005605 / NIEHS NIH HHS P01 HL091842 / NHLBI NIH HHS P01 HL051670 / NHLBI NIH HHS R01 HL112986 / NHLBI NIH HHS P30 DK054759 / NIDDK NIH HHS P30 CA086862 / NCI NIH HHS
- Language
- English
- Date published
- 01/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Pathology
- Record Identifier
- 9984051579402771
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