Approaches to analyse dynamic microbial communities such as those seen in cystic fibrosis lung

Melissa Doud; Erliang Zeng; Lisa Schneper; Giri Narasimhan; Kalai Mathee

doi:10.1186/1479-7364-3-3-246

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Approaches to analyse dynamic microbial communities such as those seen in cystic fibrosis lung

Journal article

Open access

Peer reviewed

Approaches to analyse dynamic microbial communities such as those seen in cystic fibrosis lung

Melissa Doud, Erliang Zeng, Lisa Schneper, Giri Narasimhan and Kalai Mathee

Human genomics, Vol.3(3), pp.246-256

04/2009

DOI: 10.1186/1479-7364-3-3-246

PMCID: PMC3500232

PMID: 19403459

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https://doi.org/10.1186/1479-7364-3-3-246View

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Abstract

Microbial communities play vital roles in many aspects of our lives, although our understanding of microbial biogeography and community profiles remains unclear. The number of microbes or the diversity of the microbes, even in small environmental niches, is staggering. Current microbiological methods used to analyse these communities are limited, in that many microorganisms cannot be cultured. Even for the isolates that can be cultured, the expense of identifying them definitively is much too high to be practical. Many recent molecular technologies, combined with bioinformatic tools, are raising the bar by improving the sensitivity and reliability of microbial community analysis. These tools and techniques range from those that attempt to understand a microbial community from their length heterogeneity profiles to those that help to identify the strains and species of a random sampling of the microbes in a given sample. These technologies are reviewed here, using the microbial communities present in the lungs of cystic fibrosis patients as a paradigm.

Lung - microbiology

DNA, Bacterial - genetics

Cystic Fibrosis - microbiology

Humans

RNA, Ribosomal, 16S - genetics

Genetic Techniques

Bacterial Typing Techniques - methods

Details

Title: Subtitle: Approaches to analyse dynamic microbial communities such as those seen in cystic fibrosis lung
Creators: Melissa Doud - Department of Biological Sciences, Florida International University, Miami, FL 33199, USA
Erliang Zeng
Lisa Schneper
Giri Narasimhan
Kalai Mathee
Resource Type: Journal article
Publication Details: Human genomics, Vol.3(3), pp.246-256
Publisher: England
DOI: 10.1186/1479-7364-3-3-246
PMID: 19403459
PMCID: PMC3500232
ISSN: 1473-9542
eISSN: 1479-7364
Language: English
Date published: 04/2009
Academic Unit: Preventive and Community Dentistry; Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Biostatistics; Dental Research
Record Identifier: 9984065368202771

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