Journal article
Aptamer-based detection and quantitative analysis of ricin using affinity probe capillary electrophoresis
Analytical chemistry (Washington), Vol.78(11), pp.3758-3764
06/01/2006
DOI: 10.1021/ac060021x
PMID: 16737234
Abstract
The ability to detect sub-nanomolar concentrations of ricin using fluorescently tagged RNA aptamers is demonstrated. Aptamers rival the specificity of antibodies and have the power to simplify immunoassays using capillary electrophoresis. Under nonequilibrium conditions, a dissociation constant, Kd, of 134 nM has been monitored between the RNA aptamer and ricin A-chain. With use of this free-solution assay, the detection of 500 pM (approximately 14 ng/mL) or 7.1 amol of ricin is demonstrated. The presence of interfering proteins such as bovine serum albumin and casein do not inhibit this interaction at sub-nanomolar concentrations. When spiked with RNAse A, ricin can still be detected down to 1 nM concentrations despite severe aptamer degradation. This approach offers a promising method for the rapid, selective, and sensitive detection of biowarfare agents.
Details
- Title: Subtitle
- Aptamer-based detection and quantitative analysis of ricin using affinity probe capillary electrophoresis
- Creators
- Amanda J Haes - Naval Research Laboratory, 4555 Overlook Avenue, SW, Chemistry Division, Code 6112, Washington, DC 20375-5342, USABraden C GiordanoGreg E Collins
- Resource Type
- Journal article
- Publication Details
- Analytical chemistry (Washington), Vol.78(11), pp.3758-3764
- Publisher
- United States
- DOI
- 10.1021/ac060021x
- PMID
- 16737234
- ISSN
- 0003-2700
- eISSN
- 1520-6882
- Grant note
- AI056047 / NIAID NIH HHS
- Language
- English
- Date published
- 06/01/2006
- Academic Unit
- Chemistry
- Record Identifier
- 9983985915202771
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