Apurinic/apyrimidinic endonuclease1/redox factor-1 inhibits monocyte adhesion in endothelial cells

Cuk Seong Kim; Sook Jin Son; Eun Kyung Kim; Seon Nyo Kim; Dae Goon Yoo; Hyo Shin Kim; Sung Woo Ryoo; Sang Do Lee; Kaikobad Irani; Byeong Hwa Jeon

doi:10.1016/j.cardiores.2005.10.014

Back

Apurinic/apyrimidinic endonuclease1/redox factor-1 inhibits monocyte adhesion in endothelial cells

Journal article

Open access

Peer reviewed

Apurinic/apyrimidinic endonuclease1/redox factor-1 inhibits monocyte adhesion in endothelial cells

Cuk Seong Kim, Sook Jin Son, Eun Kyung Kim, Seon Nyo Kim, Dae Goon Yoo, Hyo Shin Kim, Sung Woo Ryoo, Sang Do Lee, Kaikobad Irani and Byeong Hwa Jeon

Cardiovascular research, Vol.69(2), pp.520-526

02/01/2006

DOI: 10.1016/j.cardiores.2005.10.014

PMID: 16325162

Files and links (1)

url

https://doi.org/10.1016/j.cardiores.2005.10.014View

Published (Version of record) Open Access

Abstract

Expression of adhesion molecules on endothelial cells and subsequent monocyte adhesion are initial events in the development of atherosclerosis. The purpose of this study was to investigate the role of apurinic/apyrmidinic endonuclease1/redox factor-1 (APE1/ref-1) in the interaction of monocytes with vascular endothelial cells. Human umbilical vein endothelial cells (HUVECs) were transfected with an adenovirus encoding human APE1/ref-1. The effect of APE1/ref-1 overexpression on monocyte adhesion, vascular cell adhesion molecule-1 (VCAM-1) protein expression, and intracellular superoxide production in tumor necrosis factor (TNF)-alpha-activated HUVECs was examined. Adhesion of the monocytic cell line U937 to TNF-alpha-stimulated HUVECs in which APE1/ref-1 was overexpressed was suppressed. APE1/ref-1 overexpression also suppressed expression of VCAM-1 induced by TNF-alpha. APE1/ref-1-mediated suppression of VCAM-1 was blocked by pretreatment with the nitric oxide synthase (NOS) inhibitor l-nitroarginine methyl ester. Furthermore, APE1/ref-1 overexpression inhibited the TNF-alpha-induced increase in intracellular superoxide and p38 MAPK phosphorylation. These data provide evidence that APE1/ref-1 in endothelial cells mitigates TNF-alpha-induced monocyte adhesion and expression of vascular cell adhesion molecules, and this anti-adhesive property of APE1/ref-1 is primarily mediated by a NOS-dependent mechanism. Furthermore, APE1/ref-1 may inhibit VCAM-1 expression by inhibiting superoxide production and p38 MAPK activation.

Transfection

NG-Nitroarginine Methyl Ester - pharmacology

Monocytes - cytology

Coculture Techniques

Humans

Nitric Oxide Synthase - antagonists & inhibitors

Cell Adhesion - drug effects

Monocytes - drug effects

Tumor Necrosis Factor-alpha - pharmacology

DNA-(Apurinic or Apyrimidinic Site) Lyase - genetics

DNA-(Apurinic or Apyrimidinic Site) Lyase - metabolism

Endothelial Cells - cytology

U937 Cells

Superoxides - metabolism

p38 Mitogen-Activated Protein Kinases - metabolism

Vascular Cell Adhesion Molecule-1 - metabolism

Endothelial Cells - drug effects

Details

Title: Subtitle: Apurinic/apyrimidinic endonuclease1/redox factor-1 inhibits monocyte adhesion in endothelial cells
Creators: Cuk Seong Kim - Department of Physiology, College of Medicine, Chungnam National University, 6 Munhwa-dong, Jung-gu, Daejeon, 301-131 Korea
Sook Jin Son
Eun Kyung Kim
Seon Nyo Kim
Dae Goon Yoo
Hyo Shin Kim
Sung Woo Ryoo
Sang Do Lee
Kaikobad Irani
Byeong Hwa Jeon
Resource Type: Journal article
Publication Details: Cardiovascular research, Vol.69(2), pp.520-526
Publisher: England
DOI: 10.1016/j.cardiores.2005.10.014
PMID: 16325162
ISSN: 0008-6363
eISSN: 1755-3245
Grant note: R01 HL 70929 / NHLBI NIH HHS
Language: English
Date published: 02/01/2006
Academic Unit: Cardiovascular Medicine; Radiation Oncology; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
Record Identifier: 9984047712002771

Metrics

26 Record Views

37 Times Cited - Web of Science