Journal article
Arabidopsis thaliana PAD4 encodes a lipase-like gene that is important for salicylic acid signaling
Proceedings of the National Academy of Sciences - PNAS, Vol.96(23), pp.13583-13588
11/09/1999
DOI: 10.1073/pnas.96.23.13583
PMCID: PMC23991
PMID: 10557364
Abstract
The Arabidopsis
PAD4
gene previously was found to be required for expression of multiple defense responses including camalexin synthesis and
PR-1
gene expression in response to infection by the bacterial pathogen
Pseudomonas syringae
pv.
maculicola.
This report describes the isolation of
PAD4
. The predicted PAD4 protein sequence displays similarity to triacyl glycerol lipases and other esterases. The
PAD4
transcript was found to accumulate after
P. syringae
infection or treatment with salicylic acid (SA).
PAD4
transcript levels were very low in infected
pad4
mutants. Treatment with SA induced expression of
PAD4
mRNA in
pad4–1
,
pad4–3
, and
pad4–4
plants but not in
pad4–2
plants. Induction of
PAD4
expression by
P. syringae
was independent of the regulatory factor NPR1 but induction by SA was NPR1-dependent. Taken together with the previous observation that
pad4
mutants have a defect in accumulation of SA upon pathogen infection, these results suggest that PAD4 participates in a positive regulatory loop that increases SA levels, thereby activating SA-dependent defense responses.
Details
- Title: Subtitle
- Arabidopsis thaliana PAD4 encodes a lipase-like gene that is important for salicylic acid signaling
- Creators
- Dayadevi Jirage - Molecular and Cell Biology Graduate Program, University of Maryland, College Park, MD 20742Tina L Tootle - Molecular and Cell Biology Graduate Program, University of Maryland, College Park, MD 20742T. Lynne Reuber - Molecular and Cell Biology Graduate Program, University of Maryland, College Park, MD 20742Louise N Frost - Molecular and Cell Biology Graduate Program, University of Maryland, College Park, MD 20742Bart J Feys - Molecular and Cell Biology Graduate Program, University of Maryland, College Park, MD 20742Jane E Parker - Molecular and Cell Biology Graduate Program, University of Maryland, College Park, MD 20742Frederick M Ausubel - Molecular and Cell Biology Graduate Program, University of Maryland, College Park, MD 20742Jane Glazebrook - Molecular and Cell Biology Graduate Program, University of Maryland, College Park, MD 20742
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.96(23), pp.13583-13588
- DOI
- 10.1073/pnas.96.23.13583
- PMID
- 10557364
- PMCID
- PMC23991
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 11/09/1999
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984025568402771
Metrics
28 Record Views