Arg188 drives RhoC membrane binding

Aditi Patel; Sophia Williams-Perez; Nicole Peyton; Amy Reicks; Justin Buzick; Janean Farley; Sarah Shirar; Shawn M. Ellerbroek

doi:10.1080/21541248.2016.1205334

Back

Journal article

Open access

Peer reviewed

Arg188 drives RhoC membrane binding

Aditi Patel, Sophia Williams-Perez, Nicole Peyton, Amy Reicks, Justin Buzick, Janean Farley, Sarah Shirar and Shawn M. Ellerbroek

Small GTPases, Vol.8(2), pp.114-121

04/03/2017

DOI: 10.1080/21541248.2016.1205334

PMCID: PMC5464120

PMID: 27355867

Files and links (1)

url

https://doi.org/10.1080/21541248.2016.1205334View

Published (Version of record) Open Access

Abstract

RhoA and RhoC GTPases are 92% identical but demonstrate unique regulation and function. Phosphorylation of Ser188 has widely been reported to inhibit RhoA activity. RhoC possesses Arg188 in place of Ser188 but retains a canonical upstream PKA recognition sequence. We report here that RhoC-R188S was a PKA substrate in vitro and exhibited less GTP loading compared to wild-type RhoC when expressed in cells. Transiently expressed RhoC was found to be significantly more membrane associated than RhoA. Membrane association of RhoC-R188S and RhoC-R188A were similar to each other and wild-type RhoA, suggesting that Arg188 directly promotes RhoC membrane binding. The positive influence of Arg188 on RhoC membrane association was evident in a constitutively active (Q63L) background. In accordance, RhoA-S188R was significantly more membrane associated than either RhoA or RhoA-S188A. Altogether, these data suggest that swapping residue 188 identity effectively flips the membrane binding profile of wild-type RhoA and RhoC through positive arginine contribution rather than negative phosphoserine regulation.

arginine

membrane

RhoA

RhoC

serine

Details

Title: Subtitle: Arg188 drives RhoC membrane binding
Creators: Aditi Patel - Wartburg College
Sophia Williams-Perez - Wartburg College
Nicole Peyton - Wartburg College
Amy Reicks - Wartburg College
Justin Buzick - Wartburg College
Janean Farley - Wartburg College
Sarah Shirar - Wartburg College
Shawn M. Ellerbroek - Wartburg College
Resource Type: Journal article
Publication Details: Small GTPases, Vol.8(2), pp.114-121
DOI: 10.1080/21541248.2016.1205334
PMID: 27355867
PMCID: PMC5464120
NLM abbreviation: Small GTPases
ISSN: 2154-1248
eISSN: 2154-1256
Publisher: Taylor & Francis
Number of pages: 8
Language: English
Date published: 04/03/2017
Academic Unit: Pathology
Record Identifier: 9984966846302771

Metrics

3 Record Views

See more details