Journal article
Arginine supplementation induces myoblast fusion via augmentation of nitric oxide production
Journal of muscle research and cell motility, Vol.27(8), pp.577-584
11/2006
DOI: 10.1007/s10974-006-9078-1
PMID: 17051348
Abstract
The semi-essential amino acid, l-arginine (l-Arg), is the substrate for endogenous synthesis of nitric oxide, a molecule that is involved in myoblast proliferation and fusion. Since l-Arg supply may limit nitric oxide synthase (NOS) activity in endothelial cells, we examined l-Arg supplementation in differentiating mouse myoblasts and tested the hypothesis that l-Arg exerts direct effects on myoblast fusion via augmentation of endogenous nitric oxide production. C2C12 myoblasts in differentiation media received one of␣the␣following treatments for 120 h: 1 mM l-Arg, 0.1 mM N-nitro-l-arginine methyl ester (l-NAME), l-Arg + l-NAME, 10 mM l-Lysine, or no supplement (Control). Cultures were fixed and stained with hematoxylin and eosin for microphotometric image analysis of myotube density, nuclear density, and fusion index (% of total nuclei in myotubes). Endogenous production of nitric oxide during the treatment period peaked between 24 and 48 h. l-Arg amplified nitric oxide production between 0 and 24 h and increased myotube density, total nuclei number, and nuclear fusion index. These l-Arg effects were prevented by the NOS inhibitor, l-NAME. Further, l-Lysine, a competitive inhibitor of l-Arg uptake, repressed nitric oxide production and reduced myotube density and fusion index. In summary, l-Arg augments myotube formation and increases nitric oxide production in a process limited by cellular l-Arg uptake.
Details
- Title: Subtitle
- Arginine supplementation induces myoblast fusion via augmentation of nitric oxide production
- Creators
- Jodi Long - Center for Exercise Science, Department of Applied Physiology & Kinesiology University of Florida P.O. Box 118206 Gainesville FL 32611 USAVitor Lira - Center for Exercise Science, Department of Applied Physiology & Kinesiology University of Florida P.O. Box 118206 Gainesville FL 32611 USAQuinlyn Soltow - Center for Exercise Science, Department of Applied Physiology & Kinesiology University of Florida P.O. Box 118206 Gainesville FL 32611 USAJenna Betters - Center for Exercise Science, Department of Applied Physiology & Kinesiology University of Florida P.O. Box 118206 Gainesville FL 32611 USAJeff Sellman - Center for Exercise Science, Department of Applied Physiology & Kinesiology University of Florida P.O. Box 118206 Gainesville FL 32611 USADavid Criswell - Center for Exercise Science, Department of Applied Physiology & Kinesiology University of Florida P.O. Box 118206 Gainesville FL 32611 USA
- Resource Type
- Journal article
- Publication Details
- Journal of muscle research and cell motility, Vol.27(8), pp.577-584
- Publisher
- Kluwer Academic Publishers; Dordrecht
- DOI
- 10.1007/s10974-006-9078-1
- PMID
- 17051348
- ISSN
- 0142-4319
- eISSN
- 1573-2657
- Language
- English
- Date published
- 11/2006
- Academic Unit
- Health and Human Physiology
- Record Identifier
- 9984002348602771
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