Journal article
Arrestin-3 is essential for the activation of Fyn by the luteinizing hormone receptor (LHR) in MA-10 cells
Cellular signalling, Vol.20(10), pp.1822-1829
10/01/2008
DOI: 10.1016/j.cellsig.2008.06.005
PMID: 18647647
Abstract
Recent studies showed that Fyn is a mediator of the LHR-induced activation of the ERK1/2 cascade in MA-10 cells. Since the LHR is a G protein-coupled receptor and the Src family of kinases can be activated by some G alpha subunits and by the non-visual arrestins we investigated the role of these signaling molecules in the LHR-provoked activation of Fyn.
Small interfering RNAs (siRNAs) that target two Got subunits that participate in LHR signaling (G alpha(s) and G alpha(11)) and one that targets arrestin-3 were co-transfected with the hLHR in MA-10 cells. We then determined the effects of these siRNAs on the LHR-provoked activation of Fyn, the phosphorylation of FAK (a prominent Fyn substrate) and the release of EGF-like growth factors (a Fyn-mediated process).
Expression of the siRNA against G alpha(5) decreased the level of G alpha(5) and LHR-stimulated cAMP production by similar to 50% but did not affect LHR-stimulated Fyn activation or FAK phosphorylation. Likewise, expression of the siRNA against G alpha(11) decreased the level of Got 11 and LHR-stimulated inositol phosphate production by similar to 50% but did not affect LHR-stimulated Fyn activation or FAK phosphorylation. Expression of the siRNA against arrestin-3 decreased the level of arrestin-3 and the rate of internalization of hCG by similar to 50% and it also inhibited the LHR-provoked stimulation of Fyn, the phosphorylation of FAK and the release of EGF-like growth factors.
These results show that, in MA-10 cells, the hLHR activates Fyn through an arrestin-3-dependent pathway and that this pathway is a mediator of the hLHR-provoked release of EGF-like growth factors. (C) 2008 Elsevier Inc. All rights reserved.
Details
- Title: Subtitle
- Arrestin-3 is essential for the activation of Fyn by the luteinizing hormone receptor (LHR) in MA-10 cells
- Creators
- Colette Galet - University of IowaMario Ascoli - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Cellular signalling, Vol.20(10), pp.1822-1829
- DOI
- 10.1016/j.cellsig.2008.06.005
- PMID
- 18647647
- NLM abbreviation
- Cell Signal
- ISSN
- 0898-6568
- eISSN
- 1873-3913
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- R01 CA040629-25; R01 CA040629 / NCI NIH HHS; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) R01CA040629 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI)
- Language
- English
- Date published
- 10/01/2008
- Academic Unit
- Injury Prevention Research Center; Neuroscience and Pharmacology; University of Iowa Health Care
- Record Identifier
- 9985138030702771
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