Arsonate-specific murine T cell clones. II. Delayed-type hypersensitivity induced by P-azobenzenearsonate-L-tyrosine (ABA-Tyr)

Craig T Morita; Joel W Goodman; George K Lewis

doi:10.4049/jimmunol.134.5.2894

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Arsonate-specific murine T cell clones. II. Delayed-type hypersensitivity induced by P-azobenzenearsonate-L-tyrosine (ABA-Tyr)

Journal article

Peer reviewed

Arsonate-specific murine T cell clones. II. Delayed-type hypersensitivity induced by P-azobenzenearsonate-L-tyrosine (ABA-Tyr)

Craig T Morita, Joel W Goodman and George K Lewis

The Journal of immunology (1950), Vol.134(5), pp.2894-2899

05/1985

DOI: 10.4049/jimmunol.134.5.2894

PMID: 2580007

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Abstract

To study T cell idiotype expression at the functional level, we developed a hapten-specific delayed-type hypersensitivity (DTH) system by which we avoid the complication of anti-hapten antibody and which is specific only for the immunizing hapten, and not for conjugate specific determinants. Immunization with ABA-Tyr and challenge with ABA diazonium induced footpad swelling with the characteristics of DTH. Anti-ABA antibodies did not contribute to this reaction, as they were undetectable in mice immunized with ABA-Tyr. Furthermore, this ABA-Tyr-specific DTH was under Ir gene control identical to that reported for ABA-Tyr-specific lymphocyte proliferation. All mouse strains tested responded to ABA-Tyr except those of the b haplotype across the entire Ia region. In contrast, contact sensitivity induced by ABA diazonium was not under apparent Ir gene control, probably reflecting 1) different specificities of the induced T cells and 2) the production of anti-ABA antibodies that contribute to the footpad swelling via an Arthus reaction. Having shown that ABA-Tyr can induce T cells mediating DTH, we then examined ABA-Tyr-reactive T cell clones, propagated in vitro, for their ability to mediate DTH. Such clones elicited a response identical to that seen with in vivo immunization with respect to dose dependency, I-Ak restriction, and antigen specificity.

Hypersensitivity, Delayed - genetics

Genes, MHC Class II

p-Azobenzenearsonate - analogs & derivatives

Lymphocyte Activation

Mice, Inbred A

Azo Compounds - immunology

Mice, Inbred C57BL

Male

Epitopes

Hypersensitivity, Delayed - etiology

Hypersensitivity, Delayed - immunology

Tyrosine - analogs & derivatives

Tyrosine - immunology

Mice, Inbred C3H

Animals

Clone Cells - immunology

Immunization, Passive

Female

Mice, Inbred DBA

T-Lymphocytes - immunology

Mice

p-Azobenzenearsonate - immunology

Details

Title: Subtitle: Arsonate-specific murine T cell clones. II. Delayed-type hypersensitivity induced by P-azobenzenearsonate-L-tyrosine (ABA-Tyr)
Creators: Craig T Morita
Joel W Goodman
George K Lewis
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.134(5), pp.2894-2899
DOI: 10.4049/jimmunol.134.5.2894
PMID: 2580007
NLM abbreviation: J Immunol
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AI 05664 / NIAID NIH HHS GM 07618-02 / NIGMS NIH HHS AI 17090 / NIAID NIH HHS
Language: English
Date published: 05/1985
Academic Unit: Immunology; Internal Medicine
Record Identifier: 9984094317702771

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