Arsonate-specific murine T cell clones. III. Correlation between clonotype expression and fine specificity for analogs of L-tyrosine-p-azobenzenearsonate

Craig T Morita; William L Godfrey; Joel W Goodman; George K Lewis

doi:10.4049/jimmunol.137.7.2139

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Arsonate-specific murine T cell clones. III. Correlation between clonotype expression and fine specificity for analogs of L-tyrosine-p-azobenzenearsonate

Journal article

Peer reviewed

Arsonate-specific murine T cell clones. III. Correlation between clonotype expression and fine specificity for analogs of L-tyrosine-p-azobenzenearsonate

Craig T Morita, William L Godfrey, Joel W Goodman and George K Lewis

The Journal of immunology (1950), Vol.137(7), pp.2139-2144

10/01/1986

DOI: 10.4049/jimmunol.137.7.2139

PMID: 2428862

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Abstract

Despite recent advances in our understanding of T cell antigen receptor structure, relatively little is known about the role of this receptor in MHC-restricted antigen recognition. To study this problem, we have developed a panel of ABA-Tyr-reactive, I-Ak-restricted T cell clones that differ in their ability to recognize structural analogs of ABA-Tyr. Three fine specificity groups have been defined. In each group, ABA-Tyr elicited the strongest response of any of the antigens tested. Group I clones responded to ABA-conjugated hydroxyphenyl-ethanol (ABA-HPE). Group II clones responded to ABA-conjugated hydroxyphenyl-methanol (ABA-HPM) but not to ABA-HPE, and group III clones responded only to ABA-Tyr. These studies show that differences as small as a single methylene group can dramatically affect fine specificity. Because these clones are all I-Ak-restricted, it was possible to correlate receptor serology with fine specificity. To this end, monoclonal anti-clonotypes were made against clone 16-F2 from group I and used to study the relationship between fine specificity and clonotype expression. A panel of 15 T cell clones studied with four anti-clonotype antibodies showed a strict correlation between clonotype expression and fine specificity. Taken together, these data suggest that the structure recognized by the anti-clonotype antibodies is a determinant of receptor fine specificity.

Flow Cytometry

Tyrosine

Molecular Weight

Azo Compounds - immunology

Epitopes

Lymph Nodes - immunology

Animals

Immunoglobulin Idiotypes

Clone Cells - immunology

Receptors, Antigen, T-Cell - immunology

T-Lymphocytes - immunology

Mice

Antibodies, Monoclonal - immunology

p-Azobenzenearsonate - immunology

Details

Title: Subtitle: Arsonate-specific murine T cell clones. III. Correlation between clonotype expression and fine specificity for analogs of L-tyrosine-p-azobenzenearsonate
Creators: Craig T Morita
William L Godfrey
Joel W Goodman
George K Lewis
Resource Type: Journal article
Publication Details: The Journal of immunology (1950), Vol.137(7), pp.2139-2144
DOI: 10.4049/jimmunol.137.7.2139
PMID: 2428862
ISSN: 0022-1767
eISSN: 1550-6606
Grant note: AI-05664 / NIAID NIH HHS GM07618-02 / NIGMS NIH HHS AI-17090 / NIAID NIH HHS
Language: English
Date published: 10/01/1986
Academic Unit: Immunology; Internal Medicine
Record Identifier: 9984094480102771

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