Journal article
Arthroscopic lens distortion correction applied to dynamic cartilage loading
The Iowa orthopaedic journal, Vol.27, pp.52-57
2007
PMCID: PMC2150644
PMID: 17907430
Appears in Diamond Open Access
Abstract
It is difficult to study the deformation of articular cartilage because it is an inhomogenous material with depth dependent constituents. In many experimental studies, cartilage is assumed to behave homogeneously and is subjected to only static or quasi-static loads. In this study, a thick walled, mechanically active culture device (TRIAX) was used to apply cyclic loading to cartilage explants at physiological stress levels. An arthroscope was fitted into the wall of the TRIAX to monitorand record the cyclic compressive behavior of the cartilage and to measure depth dependent cartilage strains. A common concern with arthroscopy systems is that the images obtained are radially distorted about a central point ("fisheye" view); therefore it is necessary to correct this distortion in order to accurately quantify distances between objects within the images. To do this, an algorithm was developed which used a calibration pattern to create an image transform. Digital video of the cyclic cartilage compression was recorded, and the distortion algorithm was applied to the images to measure the cartilage as it deformed. This technique will provide valuable and practical insight into cartilage mechanics and viability (via calcein AM-stained chondrocytes) during multiday cyclic loading of living cartilage explants. The implementation of an arthroscopy system provides the advantage of bringing microscope-level resolution into a cartilage compression device to allow for digital visualization of the entire explant at the whole-tissue level.
Details
- Title: Subtitle
- Arthroscopic lens distortion correction applied to dynamic cartilage loading
- Creators
- Nicole A Kallemeyn - Department of Orthopaedics and Rehabilitation, The University of Iowa, Iowa City, IA 52242, USANicole M GroslandVincent A MagnottaJames A MartinDouglas R Pedersen
- Resource Type
- Journal article
- Publication Details
- The Iowa orthopaedic journal, Vol.27, pp.52-57
- PMID
- 17907430
- PMCID
- PMC2150644
- NLM abbreviation
- Iowa Orthop J
- ISSN
- 1541-5457
- eISSN
- 1555-1377
- Publisher
- Dept. of Orthopaedics, The University of Iowa; United States
- Grant note
- P50 AR 48939 / NIAMS NIH HHS P50 AR048939 / NIAMS NIH HHS
- Language
- English
- Date published
- 2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Radiology; Psychiatry; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Injury Prevention Research Center
- Record Identifier
- 9984040467302771
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