Journal article
Ascorbate Oxidation: UV Absorbance of Ascorbate and ESR Spectroscopy of the Ascorbyl Radical as Assays for Iron
Free radical research, Vol.10(1-2), pp.5-9
1990
DOI: 10.3109/10715769009145927
PMID: 2165985
Abstract
Catalytic transition metals are an absolute requirement for the aerobic oxidation of ascorbate monoanion. Thus, for example. the concentration of iron can be determined by the metal-dependent rate of ascorbate oxidation in near-neutral solutions. The lower limit of detection of iron, as Fe(III)EDTA. by monitoring the decrease in absorbance at 265 nm of ascorbate is about 200 nM. However, by measuring the concentration of the ascorbyl radical by ESR spectroscopy the lower limit is about 10nM.
Using these assays, I have shown that the typical microliter laboratory syringe can introduce significant iron into solutions. Thus. for studies involving iron, these two tests can be used to determine the amount of contaminating iron in reagents as well as iron from other sources such as laboratory equipment.
Details
- Title: Subtitle
- Ascorbate Oxidation: UV Absorbance of Ascorbate and ESR Spectroscopy of the Ascorbyl Radical as Assays for Iron
- Creators
- Garry R Buettner - ESR Center, EMRB 58, The University of Iowa, Iowa City, IA, 52242, USA
- Resource Type
- Journal article
- Publication Details
- Free radical research, Vol.10(1-2), pp.5-9
- Publisher
- Informa UK Ltd
- DOI
- 10.3109/10715769009145927
- PMID
- 2165985
- ISSN
- 1071-5762
- eISSN
- 1029-2470
- Language
- English
- Date published
- 1990
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047745402771
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