Journal article
Ascorbate free radical as a marker of oxidative stress: An EPR study
Free radical biology & medicine, Vol.14(1), pp.49-55
1993
DOI: 10.1016/0891-5849(93)90508-R
PMID: 8384150
Abstract
The ascorbate free radical (A
.-) is a resonance-stabilized tricarbonyl species that is readily formed from the one-electron oxidation of ascorbate, AH
−. Because of the low reduction potential of the A
.-/AH
− coupe, E°′ = +282 mV, nearly every oxidizing radical that can arise in a biological system will bring about the one-electron oxidation of AH
−, forming A.
.- Thus, the steady-state concentration of A
.- can be used as a measure of oxidative stress in chemical, biochemical, and biological systems. In this article, we discuss the energetics of ascorbate free radical reactions and demonstrate that the A
.- electron paramagnetic resonance (EPR) signal intensity can serve as a marker for the degree of oxidative stress occurring in a system.
Details
- Title: Subtitle
- Ascorbate free radical as a marker of oxidative stress: An EPR study
- Creators
- Garry R Buettner - ESR Facility/EMRB 68, The University of Iowa, Iowa City, IA 52242, USABeth Anne Jurkiewicz - ESR Facility/EMRB 68, The University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Free radical biology & medicine, Vol.14(1), pp.49-55
- DOI
- 10.1016/0891-5849(93)90508-R
- PMID
- 8384150
- NLM abbreviation
- Free Radic Biol Med
- ISSN
- 0891-5849
- eISSN
- 1873-4596
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1993
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984047710802771
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