Journal article
Aspirin Delimits Platelet Life Span by Proteasomal Inhibition
PloS one, Vol.9(8), pp.e105049-e105049
08/15/2014
DOI: 10.1371/journal.pone.0105049
PMCID: PMC4134270
PMID: 25126950
Abstract
Aspirin is widely used in clinical settings as an anti-inflammatory and anti-platelet drug due its inhibitory effect on cyclooxygenase activity. Although the drug has long been considered to be an effective and safe therapeutic regime against inflammatory and cardiovascular disorders, consequences of its cyclooxygenase-independent attributes on platelets, the key players in thrombogenesis, beg serious investigation. In this report we explored the effect of aspirin on platelet lifespan in murine model and its possible cytotoxicity against human platelets in vitro. Aspirin administration in mice led to significant reduction in half-life of circulating platelets, indicative of enhanced rate of platelet clearance. Aspirin-treated human platelets were found to be phagocytosed more efficiently by macrophages, associated with attenuation in platelet proteasomal activity and upregulation of conformationally active Bax, which were consistent with enhanced platelet apoptosis. Although the dosage of aspirin administered in mice was higher than the therapeutic regimen against cardiovascular events, it is comparable with the recommended anti-inflammatory prescription. Thus, above observations provide cautionary framework to critically re-evaluate prophylactic and therapeutic dosage regime of aspirin in systemic inflammatory as well as cardiovascular ailments.
Details
- Title: Subtitle
- Aspirin Delimits Platelet Life Span by Proteasomal Inhibition
- Creators
- Manasa K. Nayak - Banaras Hindu UniversityAyusman Dash - Indian Institute of Science Education and Research KolkataNitesh Singh - Banaras Hindu UniversityDebabrata Dash - Banaras Hindu University
- Resource Type
- Journal article
- Publication Details
- PloS one, Vol.9(8), pp.e105049-e105049
- DOI
- 10.1371/journal.pone.0105049
- PMID
- 25126950
- PMCID
- PMC4134270
- NLM abbreviation
- PLoS One
- ISSN
- 1932-6203
- eISSN
- 1932-6203
- Publisher
- Public Library Science
- Number of pages
- 9
- Grant note
- Department of Science and Technology (DST), Government of India; Department of Science & Technology (India) DBT; Department of Biotechnology (DBT) India Indian Council of Medical Research (ICMR) Council of Scientific and Industrial Research (CSIR); Council of Scientific & Industrial Research (CSIR) - India Department of Biotechnology (DBT); Department of Biotechnology (DBT) India
- Language
- English
- Date published
- 08/15/2014
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984359785602771
Metrics
24 Record Views