Journal article
Assay of purified aryl sulfotransferase suitable for reactions yielding unstable sulfuric acid esters
Analytical biochemistry, Vol.183(2), pp.320-324
1989
DOI: 10.1016/0003-2697(89)90486-7
PMID: 2624319
Abstract
An assay procedure for purified aryl sulfotransferase is described. The method utilizes isocratic paired-ion reverse-phase HPLC analysis of adenosine-3′,5′-diphosphate formed in the reaction. Evaluation of the assay procedure was carried out with 1-naphthalenemethanol as a model substrate for purified rat hepatic aryl sulfotransferase IV. Kinetic constants for sulfation of 1-naphthalenemethanol determined by this method compared favorably with those determined using thin-layer chromatographic assays of
35S incorporation. These results indicate that the method will be suitable for determination of kinetic constants in sulfotransferase-catalyzed reactions where the product sulfuric acid ester may be chemically unstable.
Details
- Title: Subtitle
- Assay of purified aryl sulfotransferase suitable for reactions yielding unstable sulfuric acid esters
- Creators
- Michael W. Duffel - University of IowaThomas P. Binder - University of IowaSatish I. Rao - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Analytical biochemistry, Vol.183(2), pp.320-324
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0003-2697(89)90486-7
- PMID
- 2624319
- ISSN
- 0003-2697
- eISSN
- 1096-0309
- Language
- English
- Date published
- 1989
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984303161402771
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