Journal article
Assembly and intracellular trafficking of HLA-B3501 and HLA-B3503
Immunogenetics (New York), Vol.61(11-12), pp.703-716
12/01/2009
DOI: 10.1007/s00251-009-0399-2
PMCID: PMC2971690
PMID: 19838694
Abstract
Residue 116 of major histocompatibility complex (MHC) class I heavy chains is an important determinant of assembly, that can influence rates of ER-Golgi trafficking, binding to the transporter associated with antigen processing (TAP), tapasin dependence of assembly, and the efficiency and specificity of peptide binding. Here, we investigated assembly and peptide-binding differences between HLA-B*3501(S116) and HLA-B*3503(F116), two alleles differing only at position 116 of the MHC class I heavy chain, that are associated respectively with normal or rapid AIDS progression. A reduced intracellular maturation rate was observed for HLA-B*3503 in HIV-infected and uninfected cells, which correlated with enhanced binding of HLA-B*3503 to TAP. No significant differences in the intrinsic efficiency of in vitro peptide binding by HLA-B*3501 and HLA-B*3503 were measurable with several common peptides or peptide libraries, and both allotypes were relatively tapasin-independent for their assembly. However, thermostability differences between the two allotypes were measurable in a CD4(+) T cell line. These findings suggest that compared to HLA-B*3501, a reduced intracellular peptide repertoire for HLA-B*3503 could contribute to its slower intracellular trafficking and stronger association with rapid AIDS progression.
Details
- Title: Subtitle
- Assembly and intracellular trafficking of HLA-B3501 and HLA-B3503
- Creators
- Vilasack Thammavongsa - University of Michigan–Ann ArborMalinda Schaefer - University of Michigan–Ann ArborTracey Filzen - University of Michigan–Ann ArborKathleen L. Collins - University of Michigan–Ann ArborMary Carrington - NCI, Canc & Inflammat Program, Expt Immunol Lab, SAIC Frederick, Frederick, MD 21702 USANaveen Bangia - Roswell Park Comprehensive Cancer CenterMalini Raghavan - University of Michigan–Ann Arbor
- Resource Type
- Journal article
- Publication Details
- Immunogenetics (New York), Vol.61(11-12), pp.703-716
- Publisher
- Springer Nature
- DOI
- 10.1007/s00251-009-0399-2
- PMID
- 19838694
- PMCID
- PMC2971690
- ISSN
- 0093-7711
- eISSN
- 1432-1211
- Number of pages
- 14
- Grant note
- AI44155 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01AI044115 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID)
- Language
- English
- Date published
- 12/01/2009
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9984696760402771
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