Journal article
Assembly of a β2-adrenergic receptor—GluR1 signalling complex for localized cAMP signalling
The EMBO journal, Vol.29(2), pp.482-495
01/20/2010
DOI: 10.1038/emboj.2009.344
PMCID: PMC2824466
PMID: 19942860
Abstract
Central noradrenergic signalling mediates arousal and facilitates learning through unknown molecular mechanisms. Here, we show that the beta(2)-adrenergic receptor (beta(2)AR), the trimeric G(s) protein, adenylyl cyclase, and PKA form a signalling complex with the AMPA-type glutamate receptor subunit GluR1, which is linked to the beta(2)AR through stargazin and PSD-95 and their homologues. Only GluR1 associated with the beta(2)AR is phosphorylated by PKA on beta(2)AR stimulation. Peptides that interfere with the beta 2AR-GluR1 association prevent this phosphorylation of GluR1. This phosphorylation increases GluR1 surface expression at postsynaptic sites and amplitudes of EPSCs and mEPSCs in prefrontal cortex slices. Assembly of all proteins involved in the classic beta(2)AR-cAMP cascade into a supramolecular signalling complex and thus allows highly localized and selective regulation of one of its major target proteins.
Details
- Title: Subtitle
- Assembly of a β2-adrenergic receptor—GluR1 signalling complex for localized cAMP signalling
- Creators
- Mei-ling A Joiner - Department of Pharmacology, University of Iowa, Iowa City, IA, USAMarie-France Lisé - Department of Psychiatry and Brain Research Center, University of British Columbia, Vancouver, British Columbia, CanadaEunice Y Yuen - Department of Physiology and Biophysics, State University of New York, Buffalo, NY, USAAngel Y F Kam - Department of Pharmacology, University of Minnesota, Minneapolis, MN, USAMingxu Zhang - Department of Pharmacology, University of Iowa, Iowa City, IA, USADuane D Hall - Department of Pharmacology, University of Iowa, Iowa City, IA, USAZulfiqar A Malik - Department of Pharmacology, University of Iowa, Iowa City, IA, USAHai Qian - Department of Pharmacology, University of Iowa, Iowa City, IA, USAYucui Chen - Department of Pharmacology, University of Iowa, Iowa City, IA, USAJason D Ulrich - Department of Pharmacology, University of Iowa, Iowa City, IA, USAAlain C Burette - Department of Cell and Developmental Biology, and Neuroscience Center, University of North Carolina, Chapel Hill, NC, USARichard J Weinberg - Department of Cell and Developmental Biology, and Neuroscience Center, University of North Carolina, Chapel Hill, NC, USAPing-Yee Law - Department of Pharmacology, University of Minnesota, Minneapolis, MN, USAAlaa El-Husseini - Department of Psychiatry and Brain Research Center, University of British Columbia, Vancouver, British Columbia, CanadaZhen Yan - Department of Physiology and Biophysics, State University of New York, Buffalo, NY, USAJohannes W Hell - Department of Pharmacology, University of Iowa, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- The EMBO journal, Vol.29(2), pp.482-495
- Publisher
- Nature Publishing Group
- DOI
- 10.1038/emboj.2009.344
- PMID
- 19942860
- PMCID
- PMC2824466
- ISSN
- 0261-4189
- eISSN
- 1460-2075
- Alternative title
- A β2AR–AMPA receptor signalling complex
- Language
- English
- Date published
- 01/20/2010
- Academic Unit
- Cardiovascular Medicine; Biology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984094623002771
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