Journal article
Assessment and modification of degenerate qPCR primers that amplify functional genes from etheneotrophs and vinyl chloride-assimilators
Letters in applied microbiology, Vol.53(5), pp.576-580
11/2011
DOI: 10.1111/j.1472-765X.2011.03144.x
PMID: 21880051
Abstract
Degenerate qPCR primer sets that target the functional genes etnC and etnE in etheneotrophs and vinyl chloride-assimilating bacteria were assessed and modified in an effort to improve performance.
Functional gene abundance in four pure cultures was estimated by qPCR using novel (MRTC and MRTE) and existing (RTC and RTE) degenerate primer sets and compared to abundances estimated with nondegenerate gene-specific primers (GSPs). Functional gene abundance in groundwater DNA extracted from several contaminated sites was also estimated with MRTC and MRTE primers.
MRTC primers displayed significantly improved etnC quantification in both pure cultures and environmental samples.
Application of MRTC and MRTE primer sets will enhance microbial ecology studies involving etheneotrophs and qPCR analyses that support vinyl chloride bioremediation strategies.
Details
- Title: Subtitle
- Assessment and modification of degenerate qPCR primers that amplify functional genes from etheneotrophs and vinyl chloride-assimilators
- Creators
- Y O Jin - Department of Civil and Environmental Engineering, 4105 Seamans Center, University of Iowa, Iowa City, IA 52242, USAT E Mattes
- Resource Type
- Journal article
- Publication Details
- Letters in applied microbiology, Vol.53(5), pp.576-580
- Publisher
- England
- DOI
- 10.1111/j.1472-765X.2011.03144.x
- PMID
- 21880051
- ISSN
- 1472-765X
- eISSN
- 1472-765X
- Language
- English
- Date published
- 11/2011
- Academic Unit
- Civil and Environmental Engineering
- Record Identifier
- 9983991949002771
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