Journal article
Assessment of resistance vessel function in human skeletal muscle: guidelines for experimental design, Doppler ultrasound, and pharmacology
American journal of physiology. Heart and circulatory physiology, Vol.318(2), pp.H301-H325
02/01/2020
DOI: 10.1152/ajpheart.00649.2019
PMCID: PMC7052621
PMID: 31886718
Abstract
The introduction of duplex Doppler ultrasound almost half a century ago signified a revolutionary advance in the ability to assess limb blood flow in humans. It is now widely used to assess blood flow under a variety of experimental conditions to study skeletal muscle resistance vessel function. Despite its pervasive adoption, there is substantial variability between studies in relation to experimental protocols, procedures for data analysis, and interpretation of findings. This guideline results from a collegial discussion among physiologists and pharmacologists, with the goal of providing general as well as specific recommendations regarding the conduct of human studies involving Doppler ultrasound-based measures of resistance vessel function in skeletal muscle. Indeed, the focus is on methods used to assess resistance vessel function and not upstream conduit artery function (i.e., macrovasculature), which has been expertly reviewed elsewhere. In particular, we address topics related to experimental design, data collection, and signal processing as well as review common procedures used to assess resistance vessel function, including postocclusive reactive hyperemia, passive limb movement, acute single limb exercise, and pharmacological interventions.
Listen to this article’s corresponding podcast at https://ajpheart.podbean.com/e/guidelines-for-doppler-ultrasound-and-resistance-vessel-function/ .
Details
- Title: Subtitle
- Assessment of resistance vessel function in human skeletal muscle: guidelines for experimental design, Doppler ultrasound, and pharmacology
- Creators
- Jacqueline K. Limberg - University of MissouriDarren P. Casey - University of IowaJoel D. Trinity - University of UtahWayne T. Nicholson - Department of Anesthesiology, Mayo Clinic, Rochester, Minnesota;D. Walter Wray - University of UtahMichael E. Tschakovsky - Queen's UniversityDaniel J. Green - The University of Western AustraliaYlva Hellsten - University of CopenhagenPaul J. Fadel - The University of Texas at ArlingtonMichael J. Joyner - Mayo ClinicJaume Padilla - University of Missouri
- Resource Type
- Journal article
- Publication Details
- American journal of physiology. Heart and circulatory physiology, Vol.318(2), pp.H301-H325
- DOI
- 10.1152/ajpheart.00649.2019
- PMID
- 31886718
- PMCID
- PMC7052621
- NLM abbreviation
- Am J Physiol Heart Circ Physiol
- ISSN
- 0363-6135
- eISSN
- 1522-1539
- Grant note
- DOI: 10.13039/100000050, name: HHS | NIH | National Heart, Lung, and Blood Institute, award: R00 HL130339, R01 HL142603, R01 HL118313, R01 HL127071, R01 HL137769; DOI: 10.13039/100000041, name: American Diabetes Association, award: Grant 1-16-ICTS-015; DOI: 10.13039/100007217, name: Health Services Research and Development, award: I01CX001999, I01RX001311; name: Natural Sciences and Engineering Research Council (NSERC) of Canada, award: Discovery Grant RGPIN/05078-2016; name: Research Tools and Instruments Grant, award: EQPEQ0407690-11; name: National Health and Medical Research Council Principal Research Fellow, award: 1080914; name: The Independent Research Fund Denmark- Medical Sciences; name: The Lundbeck Foundation and The Danish Ministry of Culture Foundation for Sport Science; DOI: 10.13039/100000050, name: HHS | NIH | National Heart, Lung, and Blood Institute, award: R35 HL139854, R01 HL137769
- Language
- English
- Date published
- 02/01/2020
- Academic Unit
- Physical Therapy and Rehabilitation Science; Fraternal Order of Eagles Diabetes Research Center
- Record Identifier
- 9984295051102771
Metrics
55 Record Views