Association between early airway damage-associated molecular patterns and subsequent bacterial infection in patients with inhalational and burn injury

Robert Maile; Samuel Jones; Yinghao Pan; Haibo Zhou; Ilona Jaspers; David B Peden; Bruce A Cairns; Terry L Noah

doi:10.1152/ajplung.00321.2014

Back

Association between early airway damage-associated molecular patterns and subsequent bacterial infection in patients with inhalational and burn injury

Journal article

Peer reviewed

Association between early airway damage-associated molecular patterns and subsequent bacterial infection in patients with inhalational and burn injury

Robert Maile, Samuel Jones, Yinghao Pan, Haibo Zhou, Ilona Jaspers, David B Peden, Bruce A Cairns and Terry L Noah

American journal of physiology. Lung cellular and molecular physiology, Vol.308(9), pp.L855-L860

05/01/2015

DOI: 10.1152/ajplung.00321.2014

PMCID: PMC4421787

PMID: 25770180

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/4421787View

Open Access

Abstract

Bacterial infection is a major cause of morbidity affecting outcome following burn and inhalation injury. While experimental burn and inhalation injury animal models have suggested that mediators of cell damage and inflammation increase the risk of infection, few studies have been done on humans. This is a prospective, observational study of patients admitted to the North Carolina Jaycee Burn Center at the University of North Carolina who were intubated and on mechanical ventilation for treatment of burn and inhalational injury. Subjects were enrolled over a 2-yr period and followed till discharge or death. Serial bronchial washings from clinically indicated bronchoscopies were collected and analyzed for markers of tissue injury and inflammation. These include damage-associated molecular patterns (DAMPs) such as hyaluronic acid (HA), double-stranded DNA (dsDNA), heat-shock protein 70 (HSP-70), and high-mobility group protein B-1 (HMGB-1). The study population was comprised of 72 patients who had bacterial cultures obtained for clinical indications. Elevated HA, dsDNA, and IL-10 levels in bronchial washings obtained early (the first 72 h after injury) were significantly associated with positive bacterial respiratory cultures obtained during the first 14 days postinjury. Independent of initial inhalation injury severity and extent of surface burn, elevated levels of HA dsDNA and IL-10 in the central airways obtained early after injury are associated with subsequent positive bacterial respiratory cultures in patients intubated after acute burn/inhalation injury.

Adult

Bacterial Infections - pathology

Biomarkers - metabolism

Bronchoscopy

Burns, Inhalation - metabolism

DNA - metabolism

Female

HMGB1 Protein - metabolism

HSP70 Heat-Shock Proteins - metabolism

Humans

Hyaluronic Acid - metabolism

Interleukin-10 - metabolism

Lung - pathology

Lung Injury - pathology

Male

Middle Aged

Prospective Studies

Respiration, Artificial

Details

Title: Subtitle: Association between early airway damage-associated molecular patterns and subsequent bacterial infection in patients with inhalational and burn injury
Creators: Robert Maile - University of North Carolina at Chapel Hill
Samuel Jones - University of North Carolina at Chapel Hill
Yinghao Pan - University of North Carolina at Chapel Hill
Haibo Zhou - University of North Carolina at Chapel Hill
Ilona Jaspers - University of North Carolina at Chapel Hill
David B Peden - University of North Carolina at Chapel Hill
Bruce A Cairns - University of North Carolina at Chapel Hill
Terry L Noah - University of North Carolina at Chapel Hill
Resource Type: Journal article
Publication Details: American journal of physiology. Lung cellular and molecular physiology, Vol.308(9), pp.L855-L860
DOI: 10.1152/ajplung.00321.2014
PMID: 25770180
PMCID: PMC4421787
NLM abbreviation: Am J Physiol Lung Cell Mol Physiol
ISSN: 1040-0605
eISSN: 1522-1504
Grant note: KL2TR001109 / NCATS NIH HHS UL1 TR002489 / NCATS NIH HHS 1UL1TR0011111 / NCATS NIH HHS P30 ES010126 / NIEHS NIH HHS KL2 TR001109 / NCATS NIH HHS K08 GM109106 / NIGMS NIH HHS
Language: English
Date published: 05/01/2015
Academic Unit: Surgery
Record Identifier: 9984755390502771

Metrics

18 Record Views

31 Times Cited - Web of Science