Journal article
Association between enhanced TH2/TH1 cytokine profile and donor T-cell chimerism following total lymphoid irradiation
Human immunology, Vol.52(2), pp.144-154
1997
DOI: 10.1016/S0198-8859(96)00291-1
PMID: 9077563
Abstract
Total lymphoid irradiated (TLI) mice develop antigen specific tolerance if the initial antigen exposure occurs shortly after the completion of TLI. We injected TLI-treated mice with semiallogeneic donor cells at 2, 7, or 28 days after completing TLI and determined the levels of donor CD4 and CD8 cells 5 to 7 weeks after TLI treatment. The level of chimerism correlated with the timing of the initial alloantigen exposure. Donor CD4 and CD8 cells were noted only in day 2 or 7 injected mice. Because donor cell chimerism suggested increased
in vivo survival of donor cells, we used the level of donor cell chimerism as a surrogate marker for tolerance to examine the relationship between the development of tolerance and enhanced Th2/Th1 cytokine responses to donor antigen. Increased levels of donor CD4 and CD8 cells in the TLI-treated mice was associated with increased Th2/Th1 cytokine production and decreased CTL activity to donor antigen
in vitro. Higher Th2/Th1 cytokine levels also correlated with lower CTL activity. The results indicate that the increased production of Th2/Th1 may function to enhance survival of donor cells in TLI-treated mice and suggest that tolerance induction after TLI treatment involves immunoredirection.
Details
- Title: Subtitle
- Association between enhanced TH2/TH1 cytokine profile and donor T-cell chimerism following total lymphoid irradiation
- Creators
- Elizabeth H Field - From the Department of Veterans Affairs Medical Center, Iowa City, USATodd M Rouse - From the Department of Veterans Affairs Medical Center, Iowa City, USAQinglin Gao - From the Department of Medicine, University of Iowa College of Medicine, Iowa City, Iowa, U.S.ABrice Chang - From the Department of Medicine, University of Iowa College of Medicine, Iowa City, Iowa, U.S.A
- Resource Type
- Journal article
- Publication Details
- Human immunology, Vol.52(2), pp.144-154
- DOI
- 10.1016/S0198-8859(96)00291-1
- PMID
- 9077563
- NLM abbreviation
- Hum Immunol
- ISSN
- 0198-8859
- eISSN
- 1879-1166
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1997
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094643202771
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